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Table 1 Chromatographic parameters and performance data of the proposed HPLC method for determination of LND in plasma

From: Trace determination of lenalidomide in plasma by non-extractive HPLC procedures with fluorescence detection after pre-column derivatization with fluorescamine

Parameter

Value

Retention time for LND-FLC complex (min)

11.6

Retention time of the nearest adjacent peak (min)

9.1

Capacity factor (K’) for LND-FLC complex

8.4

Resolution between LND-FLC complex and the adjacent peak

3.5

LND-FLC complex peak asymmetry factor at 10% peak height

1.33

Number of theoretical plates (N) per meter

13764

Height equivalent to theoretical plate (μm)

72.6

Correlation coefficient

0.9995

Slope (b) ± SD

3.11 × 105 ± 5.89 × 103

Intercept (a) ± SD

1.272009× 105 ± 7.7 × 104

Linearity range (ng/mL)

5 – 100

LOD (ng/mL)

0.8

LOQ (ng/mL)

2.3