Figure 3

Effect of HP-β-CD concentration (A), pH (B), SDS concentration (C), phosphate concentration (D), temperature (E) and applied voltage (F) on resolutions between hypaphorine and THSG (RHT, “blue circle symbol”), proanthocyanidin B1 and catechin (RPC, “red square symbol”), and analytical time calculated as the retention time of gallic acid (R _GA , “green triangle symbol”). Default MEKC condition: Pressure injection 25 mbar, 3 s. Running buffer containing 90 mM SDS, 50 mM phosphate and 2% HP-β-CD (pH=2.5); voltage, 15 kV; temperature, 20°C; UV detection at 210 nm.