Application of smart spectrophotometric methods and artificial neural network for the simultaneous quantitation of olmesartan medoxamil, amlodipine besylate and hydrochlorothiazide in their combined pharmaceutical dosage form

Table 5 Statistical comparison for the results obtained by the proposed methods and the reported method for the analysis of AML, OLM and HCT in Tribenzor® tablets (Batch No. 134809)

Concentration (μg mL^-1)			Spectrophotometric methods			ANN method			Reported method ^a
			Ratio subtraction	Isoabsorptive	Direct	ANN method			Reported method ^a
			Recovery %
AML	OLM	HCT	AML	OLM	HCT	AML	OLM	HCT	AML	OLM	HCT
6.95	20	12.5	98.5	99.19	97.66	99.04	98.6	99.33	98.79	98.33	98.42
6.95	20	12.5	98.5	98.98	97.18	100.08	96.86	97	98.46	97.87	98.07
6.95	20	12.5	98.49	98.67	96.91	99.28	97.47	96.88	99.62	97.79	97.69
6.95	20	12.5	98.51	98.20	96.36	100.48	98.28	97.34	98.28	97.13	97.27
6.95	20	12.5	98.5	97.87	96.6	100.4	95.64	98.08	98.44	97.17	97.24
6.95	20	12.5	97.15	96.94	95.99	96.96	97.54	96.01	97.78	96.42	96.33
Mean			98.28	98.31	97.12	99.37	97.39	97.44	98.56	97.45	97.50
SD			0.552	0.829	0.587	1.321	1.061	1.144	0.615	0.678	0.736
RSD%			0.562	0.843	0.604	1.329	1.089	1.174	0.624	0.696	0.755
n			6	6	6	6	6	6	6	6	6
Variance			0.305	0.687	0.345	1.745	1.126	1.309	0.378	0.46	0.542
Student's t test^b			0.842 (2.228)	1.955 (2.228)	0.994 (2.228)	1.608 (2.228)	1.355 (2.228)	1.73 (2.228)	-----	-----	-----
F value ^b			1.241 (5.050)	1.497 (5.050)	1.574 (5.050)	4.307 (5.050)	3.338 (5.050)	1.574 (5.050)	-----	-----	-----

^a Reference spectrophotometric method is that published in the literature [13].
^b The values in the parenthesis are the corresponding theoretical values of t and F at P = 0.05.

ISSN: 2661-801X