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Table 3 Comparison of OH-PCBs atropisomer levels and enantiomeric fractions (EF) determined by LC-MS and GC-ECD methods

From: Atropisomeric determination of chiral hydroxylated metabolites of polychlorinated biphenyls using HPLC-MS

Sample Congener LC-MS GC-ECD GC-ECD
Nucleodex β-PM BDM chiral column DB1 achiral column
PCB91 E1-5OH-PCB91 (μg mL-1) 0.306 ± 0.011 0.268 ± 0.006 1.334 ± 0.023
E2-5OH-PCB91 (μg mL-1) 1.364 ± 0.068 1.160 ± 0.038
Total (μg mL-1) 1.670 ± 0.079 1.428 ± 0.044
EF (5OH-PCB91) 0.183 ± 0.002 0.188 ± 0.001  
4OH-PCB91 ND ND 0.013 ± 0.001
Recovery of 4OH-PCB159 (%) 82.3 ± 4.3 97.3 ± 1.7 99.9 ± 3.6
PCB95 E1-5OH-PCB95 (μg mL-1) ND 0.262 ± 0.014 1.183 ± 0.068
E2-5OH-PCB95 (μg mL-1) 1.346 ± 0.345 0.789 ± 0.042
Total (μg mL-1) 1.346 ± 0.345 1.051 ± 0.057
EF (5OH-PCB95)   0.249 ± 0.001  
4OH-PCB95 ND ND 0.007 ± 0.002
Recovery of 4OH-PCB159 (%) 96.5 ± 9.0 106.6 ± 11.4 101.5 ± 9.1
PCB149 E1-5OH-PCB149 (μg mL-1) 0.913 ± 0.079 0.648 ± 0.019 1.281 ± 0.050
E2-5OH-PCB149 (μg mL-1) 0.335 ± 0.029 0.272 ± 0.009
Total (μg mL-1) 1.248 ± 0.108 0.920 ± 0.028
EF(5OH-PCB149) 0.731 ± 0.007 0.704 ± 0.003  
Recovery of 4OH-PCB159 (%) 88.6 ± 0.7 113.8 ± 1.9 115.4 ± 1.2
Blank control Any OH-PCBs ND ND ND
Recovery of 4OH-PCB159 (%) 92.5 - 111
  1. ND: not detected.