Table 3 Comparison of OH-PCBs atropisomer levels and enantiomeric fractions (EF) determined by LC-MS and GC-ECD methods
Sample | Congener | LC-MS | GC-ECD | GC-ECD |
|---|---|---|---|---|
Nucleodex β-PM | BDM chiral column | DB1 achiral column | ||
PCB91 | E1-5OH-PCB91 (μg mL-1) | 0.306 ± 0.011 | 0.268 ± 0.006 | 1.334 ± 0.023 |
E2-5OH-PCB91 (μg mL-1) | 1.364 ± 0.068 | 1.160 ± 0.038 | ||
Total (μg mL-1) | 1.670 ± 0.079 | 1.428 ± 0.044 | ||
EF (5OH-PCB91) | 0.183 ± 0.002 | 0.188 ± 0.001 | ||
4OH-PCB91 | ND | ND | 0.013 ± 0.001 | |
Recovery of 4OH-PCB159 (%) | 82.3 ± 4.3 | 97.3 ± 1.7 | 99.9 ± 3.6 | |
PCB95 | E1-5OH-PCB95 (μg mL-1) | ND | 0.262 ± 0.014 | 1.183 ± 0.068 |
E2-5OH-PCB95 (μg mL-1) | 1.346 ± 0.345 | 0.789 ± 0.042 | ||
Total (μg mL-1) | 1.346 ± 0.345 | 1.051 ± 0.057 | ||
EF (5OH-PCB95) | 0.249 ± 0.001 | |||
4OH-PCB95 | ND | ND | 0.007 ± 0.002 | |
Recovery of 4OH-PCB159 (%) | 96.5 ± 9.0 | 106.6 ± 11.4 | 101.5 ± 9.1 | |
PCB149 | E1-5OH-PCB149 (μg mL-1) | 0.913 ± 0.079 | 0.648 ± 0.019 | 1.281 ± 0.050 |
E2-5OH-PCB149 (μg mL-1) | 0.335 ± 0.029 | 0.272 ± 0.009 | ||
Total (μg mL-1) | 1.248 ± 0.108 | 0.920 ± 0.028 | ||
EF(5OH-PCB149) | 0.731 ± 0.007 | 0.704 ± 0.003 | ||
Recovery of 4OH-PCB159 (%) | 88.6 ± 0.7 | 113.8 ± 1.9 | 115.4 ± 1.2 | |
Blank control | Any OH-PCBs | ND | ND | ND |
Recovery of 4OH-PCB159 (%) | 92.5 | - | 111 |