Condition | Time table of gradient mobile phases |
---|---|
I | 0 min (0.3 mL/min, A:B = 55:45) → 25 min (0.3 mL/min, A:B = 55:45) → 26 min (0.2 mL/min, A:B = 50:50) → 55 min (0.2 mL/min, A:B = 50:50) → 56 min (0.2 mL/min, A:B = 45:55) → 65 min (0.2 mL/min, A:B = 45:55) → 66 min (0.35 mL/min, A:B = 45:55) → 89 min (0.35 mL/min, A:B = 45:55) → 90 min (0.35 mL/min, A:B = 55:45) → Total 100 min |
II | 0 min (0.2 mL/min, A:B = 55:45) → 25 min (0.2 mL/min, A:B = 55:45) → 26 min (0.2 mL/min, A:B = 50:50) → 55 min (0.2 mL/min, A:B = 50:50) → 56 min (0.2 mL/min, A:B = 45:55) → 65 min (0.2 mL/min, A:B = 45:55) → 66 min (0.35 mL/min, A:B = 45:55) → 89 min (0.35 mL/min, A:B = 45:55) → 90 min (0.35 mL/min, A:B = 55:45) → Total 100 min |
III | 0 min (0.2 mL/min, A:B = 55:45) → 25 min (0.2 mL/min, A:B = 55:45) → 26 min (0.2 mL/min, A:B = 50:50) → 55 min (0.2 mL/min, A:B = 50:50) → 56 min (0.2 mL/min, A:B = 45:55) → 60 min (0.2 mL/min, A:B = 45:55) → 61 min (0.35 mL/min, A:B = 45:55) → 89 min (0.35 mL/min, A:B = 45:55) → 90 min (0.35 mL/min, A:B = 55:45) → Total 100 min |