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Table 4 Process variables and their levels used in CCD

From: Ultrasound-assisted lipase-catalyzed synthesis of D-isoascorbyl palmitate: process optimization and Kinetic evaluation

   Levels
Independent variables Coded symbols -2 -1 0 1 2
Temperature (°C) A(X1) 30 40 50 60 70
Molar ratio (D-isoascorbic: palmitic acid) B(X2) 1:1 1:2 1:3 1:4 1:5
Enzyme load (w/w) C(X3) 0.5 5 9.5 14 18.5
Ultrasonic power (W) D(X4) 60 90 120 150 180