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Table 3 Effect of ions and protease inhibitors on the trypsin of crevalle jack pyloric caeca

From: Metal-sensitive and thermostable trypsin from the crevalle jack (Caranx hippos) pyloric caeca: purification and characterization

Ion and inhibitor Residual activity ± SD (%)
Control* 100.0 ± 0.6a
Ions (1 mM)  
Cd2+ 0.31 ± 0.1b
Al3+ 0.42 ± 0.0b
Zn2+ 17.7 ± 0.5c
Cu2+ 23.8 ± 1.2d
Pb2+ 38.6 ± 0.6e
Hg2+ 44.9 ± 0.7f
Co2+ 53.8 ± 0.1g
K+ 63.3 ± 0.8h
Li+ 65.4 ± 1.3h
Ba2+ 67.4 ± 1.7h
Mn2+ 69.4 ± 0.2h
Mg2+ 75.4 ± 0.9i
Ca2+ 78.9 ± 1.7i
Inhibitors (1 mM)  
PMSF 22.4 ± 1.7b
TLCK 0 ± 0c
TPCK 100.0 ± 1.2a
Benzamidine 1.43 ± 0.2d
EDTA 100.0 ± 0.7a
  1. The results are represented by mean ± standard deviation. *Activity without ions or inhibitors addition. Different superscript letters represent statistical differences (p < 0.05, n = 3).