BMC Chemistry

Table 3 Effect of ions and protease inhibitors on the trypsin of crevalle jack pyloric caeca

From: Metal-sensitive and thermostable trypsin from the crevalle jack (Caranx hippos) pyloric caeca: purification and characterization

Ion and inhibitor	Residual activity ± SD (%)
Control*	100.0 ± 0.6^a
Ions (1 mM)
Cd²⁺	0.31 ± 0.1^b
Al³⁺	0.42 ± 0.0^b
Zn²⁺	17.7 ± 0.5^c
Cu²⁺	23.8 ± 1.2^d
Pb²⁺	38.6 ± 0.6^e
Hg²⁺	44.9 ± 0.7^f
Co²⁺	53.8 ± 0.1^g
K⁺	63.3 ± 0.8^h
Li⁺	65.4 ± 1.3^h
Ba²⁺	67.4 ± 1.7^h
Mn²⁺	69.4 ± 0.2^h
Mg²⁺	75.4 ± 0.9ⁱ
Ca²⁺	78.9 ± 1.7ⁱ
Inhibitors (1 mM)
PMSF	22.4 ± 1.7^b
TLCK	0 ± 0^c
TPCK	100.0 ± 1.2^a
Benzamidine	1.43 ± 0.2^d
EDTA	100.0 ± 0.7^a

The results are represented by mean ± standard deviation. *Activity without ions or inhibitors addition. Different superscript letters represent statistical differences (p < 0.05, n = 3).

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ISSN: 2661-801X

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