Table 3 Analsysis of the recovery of ten compounds in Shen-Fu decoction
Compounds | Initial amount(ng) | Added amount(ng) | Detected amount(ng) | Recovery (%) | RSD/% (n=5) |
|---|---|---|---|---|---|
Ginsenosid-Re | 2074.12 | 1700 | 3673.54 ± 230.37 | 94.08 | 6.27 |
|  | 2074.12 | 2100 | 3991.27 ± 117.73 | 91.29 | 2.95 |
|  | 2074.12 | 2500 | 4475.56 ± 236.76 | 96.06 | 5.29 |
Ginsenoside-Rg1 | 2260.51 | 1800 | 3959.88 ± 307.37 | 94.41 | 7.76 |
|  | 2260.51 | 2250 | 4310.87 ± 229.80 | 91.13 | 5.33 |
|  | 2260.51 | 2700 | 5176.34 ± 166.16 | 107.99 | 3.21 |
Ginsenoside-Rb1 | 2423.46 | 2000 | 4299.39 ± 196.65 | 93.80 | 4.57 |
|  | 2423.46 | 2500 | 4812.75 ±185.92 | 95.57 | 3.86 |
|  | 2423.46 | 3000 | 5271.72 ± 130.37 | 94.94 | 2.47 |
Ginsenoside-Rc | 2231.62 | 1800 | 3933.62 ± 155.38 | 94.56 | 3.95 |
|  | 2231.62 | 2250 | 4644.8 ± 231.31 | 107.25 | 4.98 |
|  | 2231.62 | 2700 | 5043.67 ± 244.21 | 104.15 | 4.84 |
Ginsenoside-Rb2 | 1597.95 | 1200 | 2880.31 ± 83.82 | 106.86 | 2.91 |
|  | 1597.95 | 1500 | 3277.51 ± 128.48 | 111.97 | 3.92 |
|  | 1597.95 | 1800 | 3510.26 ± 169.97 | 106.24 | 4.84 |
Ginsenoside-Rd | 816.73 | 640 | 1416.19 ± 86.39 | 93.67 | 6.10 |
|  | 816.73 | 800 | 1642.99 ± 95.82 | 103.28 | 5.83 |
|  | 816.73 | 960 | 1700.19 ± 80.93 | 92.03 | 4.76 |
Ginsenoside-Rf | 2000.12 | 1600 | 3563.83 ± 252.68 | 97.73 | 7.09 |
|  | 2000.12 | 2000 | 4173.63 ± 261.27 | 108.68 | 6.26 |
|  | 2000.12 | 2400 | 4549.1 ± 256.57 | 106.21 | 5.64 |
Aconitine | 2.91 | 2.4 | 5.52 ± 0.27 | 108.75 | 4.89 |
|  | 2.91 | 3.0 | 5.67 ± 0.31 | 92.00 | 5.47 |
|  | 2.91 | 3.6 | 6.7 ± 0.34 | 105.28 | 5.07 |
Mesaconitine | 7.12 | 5.6 | 12.98 ± 0.66 | 104.64 | 5.08 |
|  | 7.12 | 7.0 | 13.74 ± 0.68 | 94.57 | 4.95 |
|  | 7.12 | 8.4 | 15.82 ± 0.56 | 103.57 | 3.54 |
Hypaconitine | 100.06 | 80 | 174.69 ± 7.95 | 93.29 | 4.55 |
|  | 100.06 | 100 | 204.9 ± 10.90 | 104.84 | 5.32 |
|  | 100.06 | 120 | 225.07 ± 7.81 | 104.18 | 3.47 |