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Table 1 Effect of experimental parameters on the number of theoretical plates, resolution and selectivity factor

From: Micellar liquid chromatographic method for the simultaneous determination of Levofloxacin and Ambroxol in combined tablets: Application to biological fluids

Parameter

 

Number of theoretical plates/m (N)

Resolution (R)

Selectivity factor(α)

LEV

AMB

% concentration of organic modifier (v/v)

6

3390

5000

2.27

1.85

8

3800

6880

3.81

2.05

10

5620

3200

2.76

2.45

12

3590

5350

2.98

2.42

14

3350

6000

3.10

2.67

Concentration of SDS, M

0.05

4550

8250

5.54

2.68

0.10

4390

6500

3.81

2.45

0.12

4160

5730

3.14

2.20

0.15

5610

3190

3.81

2.45

0.16

3910

4960

2.44

2.09

0.18

2750

3310

1.90

2.11

0.20

2910

4570

1.75

1.84

pH of the medium

3

4630

7130

3.90

2.35

4

4390

6500

3.81

2.45

5

3370

6550

3.80

2.46

5.5

3980

7340

3.57

2.35

6

3160

6730

3.58

2.31

7

1360

8440

2.99

2.31

Flow rate (mL/min)

0.6

3530

5210

2.09

1.90

 

0.8

2960

4900

2.06

1.83

 

1.0

4390

6500

3.81

2.45

 

1.2

2790

3660

1.92

1.84

 

1.4

2100

3340

1.89

1.85

 

1.6

2230

3380

1.85

1.85

 

1.8

1370

3550

2.10

1.88