Parameter | Number of theoretical plates/m (N) | Resolution (R) | Selectivity factor(α) | ||
---|---|---|---|---|---|
LEV | AMB | ||||
% concentration of organic modifier (v/v) | 6 | 3390 | 5000 | 2.27 | 1.85 |
8 | 3800 | 6880 | 3.81 | 2.05 | |
10 | 5620 | 3200 | 2.76 | 2.45 | |
12 | 3590 | 5350 | 2.98 | 2.42 | |
14 | 3350 | 6000 | 3.10 | 2.67 | |
Concentration of SDS, M | 0.05 | 4550 | 8250 | 5.54 | 2.68 |
0.10 | 4390 | 6500 | 3.81 | 2.45 | |
0.12 | 4160 | 5730 | 3.14 | 2.20 | |
0.15 | 5610 | 3190 | 3.81 | 2.45 | |
0.16 | 3910 | 4960 | 2.44 | 2.09 | |
0.18 | 2750 | 3310 | 1.90 | 2.11 | |
0.20 | 2910 | 4570 | 1.75 | 1.84 | |
pH of the medium | 3 | 4630 | 7130 | 3.90 | 2.35 |
4 | 4390 | 6500 | 3.81 | 2.45 | |
5 | 3370 | 6550 | 3.80 | 2.46 | |
5.5 | 3980 | 7340 | 3.57 | 2.35 | |
6 | 3160 | 6730 | 3.58 | 2.31 | |
7 | 1360 | 8440 | 2.99 | 2.31 | |
Flow rate (mL/min) | 0.6 | 3530 | 5210 | 2.09 | 1.90 |
0.8 | 2960 | 4900 | 2.06 | 1.83 | |
1.0 | 4390 | 6500 | 3.81 | 2.45 | |
1.2 | 2790 | 3660 | 1.92 | 1.84 | |
1.4 | 2100 | 3340 | 1.89 | 1.85 | |
1.6 | 2230 | 3380 | 1.85 | 1.85 | |
1.8 | 1370 | 3550 | 2.10 | 1.88 |