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Table 1 Effect of experimental parameters on the number of theoretical plates, resolution and selectivity factor

From: Micellar liquid chromatographic method for the simultaneous determination of Levofloxacin and Ambroxol in combined tablets: Application to biological fluids

Parameter   Number of theoretical plates/m (N) Resolution (R) Selectivity factor(α)
LEV AMB
% concentration of organic modifier (v/v) 6 3390 5000 2.27 1.85
8 3800 6880 3.81 2.05
10 5620 3200 2.76 2.45
12 3590 5350 2.98 2.42
14 3350 6000 3.10 2.67
Concentration of SDS, M 0.05 4550 8250 5.54 2.68
0.10 4390 6500 3.81 2.45
0.12 4160 5730 3.14 2.20
0.15 5610 3190 3.81 2.45
0.16 3910 4960 2.44 2.09
0.18 2750 3310 1.90 2.11
0.20 2910 4570 1.75 1.84
pH of the medium 3 4630 7130 3.90 2.35
4 4390 6500 3.81 2.45
5 3370 6550 3.80 2.46
5.5 3980 7340 3.57 2.35
6 3160 6730 3.58 2.31
7 1360 8440 2.99 2.31
Flow rate (mL/min) 0.6 3530 5210 2.09 1.90
  0.8 2960 4900 2.06 1.83
  1.0 4390 6500 3.81 2.45
  1.2 2790 3660 1.92 1.84
  1.4 2100 3340 1.89 1.85
  1.6 2230 3380 1.85 1.85
  1.8 1370 3550 2.10 1.88