Figure 4From: Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretionTrx-1 distribution to the membrane is impaired by buthionine sulfoximine (BSO). Biotinylated plasma membrane surface proteins from Jurkat T cells without (C, CON) and with (BSO) glutathione depletion, were purified by streptavidin beads and characterised using antibodies to detect plasma membrane (CD3) and cytosol (lactate dehydrogenase, LDH) proteins and thioredoxin (Trx-1).Back to article page