Measurement of Ames mutation fold increases presented as a function of deposited mass which was captured in situ of exposure [a] and as a Log
conversion [b] following a 24 minute (3 cigarettes) 3R4F smoke exposure period. [a] Average deposited mass values for a 24 minute exposure for airflows 1.0, 4.0, 8.0 and 12.0 L/min were 2.30 ± 0.14, 0.50 ± 0.10, 0.09 ± 0.02 and 0.03 ± 0.01 μg/cm2 respectively. [b] Using a Log10 conversion and regression analysis fold mutation frequencies showed a positive correlation with deposited mass obtained concurrently with biological data (R2 = 0.763) with confidence intervals (red dash) and probability intervals (grey dash) of 95%. Results are based on three independent experiments.