Table 4 Validation metrics results of the SIC-FL method
From: Native fluorescent detection with sequential injection chromatography for doping control analysis
Analytical character | Result | |
|---|---|---|
Resolution | 8.2 | |
Consumed mobile phase volume (mL) | 4.5 | |
Total analysis time (min) | 4.7 | |
Sample frequency (samples/h) | 13 | |
AML | FSM | |
Retention time (min) | 0.85 | 1.62 |
Peak symmetry | 1.00 | 0.89 |
Theoretical plates | 671.55 | 937.95 |
Regression equation | PAa = 1.6387Cb + 566.28 | PAa = 26.667Cb + 23.333 |
Correlation coefficient | 0.9971 | 0.9987 |
Linear range (μg/mL) | 0.1-4.0 | 2.0-10.0 |
Within-run precision (RSD, %) | 1.98 | 2.02 |
Between-run precision (RSD, %) | 0.57 | 2.81 |
Recovery in urine samples (%) | 89 | 91 |
Recovery in tablets (%) | 99.1 for 1.0 μg/mL | 98.7 for 8.0 μg/mL |
97.5 for 0.5 μg/mL | 98.1 for 4.0 μg/mL | |
97.0 for 0.5 μg/mL | 96.5 for 0.5 μg/mL | |
LOD (μg/mL) | 0.012 | 0.470 |
LOQ (μg/mL) | 0.060 | 1.500 |