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Table 2 2 3 full-factorial design matrix a and further experiments b for screening the effect of pH buffer concentration and percentage volume of acetonitrile on resolution (R), retention time (t R , min) and peak area

From: Native fluorescent detection with sequential injection chromatography for doping control analysis

pH

BCa

ACN%

R

tR

Peak area

    

AML

FSM

AML

FSM

4.0

25

30

< 1.0

-

-

-

-

4.0

25

70

8.5

0.89

0.95

975161

990962

4.0

10

30

< 1.0

-

-

-

-

4.0

10

70

8.5

0.89

0.94

986511

981150

3.0

25

30

< 1.0

-

-

-

-

3.0

25

70

8.7

2.25

2.37

2908429

6670422

3.0

10

30

< 1.0

-

-

-

-

3.0

10

70

8.2

2.35

2.43

931563

1070808

4.0

25

50

6.9

0.78

1.06

1044527

1072148

4.0

25

60

7.8

0.84

1.20

1007888

750986

4.0

25

65

8.2

0.85

1.62

1032450

901919

  1. athe first eight rows compile the 23 full-factorial design matrix; bthe last three rows compile further experiments.