Table 5 Summary of validation parameters
Parameter | Data of standard stanazolol |
|---|---|
(at λmax750 nm) | |
Linearity range | 200-1200 ng/spot |
Correlation coefficient | 0.9990 ± 0.0004 |
Limit of detection (LOD) | 1.6 ng/spot |
Limit of quantification (LOQ) | 5.1 ng/spot |
Y = mx + c | Y = 5.841x + 1420.3 |
Slope ± SD | 5.841 ± 0.755 |
Intercept ± SD | 1420.3 ± 36.4 |
Intra-day analysis (n=3), | 0.690 |
% R.S.D. | |
Inter-day analysis (n=3), | 0.877 |
% R.S.D. | |
Robustness | Robust |
Specificity | Specific |