Table 3 Robustness testing (n = 6)
Standard level | Mobile phase composition | Nature of TLC | Time from spotting to chromatography | Time from chromatography to staining | Time for staining | Time from staining to scanning | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Amount (ng/spot-1) | Amount detected (ng ± SD) | R.S.D. (%) | Amount detected (ng ± S.D.) | R.S.D. (%) | Amount detected (ng ± SD) | R.S.D. (%) | Amount detected (ng ± SD) | R.S.D. (%) | Amount detected (ng ± SD) | R.S.D. (%) | Amount detected (ng ± SD) | R.S.D. (%) |
300 | 303.7 ± 11.54 | 3.80 | 298.3 ± 1.54 | 0.51 | 311.29 ± 8.03 | 2.58 | 284.18 ± 6.81 | 2.39 | 300.63 ± 9.34 | 3.11 | 287.3 ± 10.06 | 3.5 |
500 | 505.01 ±12.44 | 2.46 | 500.7 ±13.58 | 2.71 | 498.51 ± 18.85 | 3.78 | 513.85 ± 6.91 | 1.34 | 516.18 ± 2.58 | 0.50 | 508.53 ± 12.96 | 2.54 |
700 | 705.23 ± 6.64 | 0.94 | 698.46 ± 6.64 | 0.95 | 717.03 ± 3.43 | 0.47 | 699.61 ± 0.51 | 0.07 | 714.95 ± 12.30 | 1.72 | 704.29 ± 3.33 | 0.47 |