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Table 1 Validation of the method

From: Photodegradation assessment of ciprofloxacin, moxifloxacin, norfloxacin and ofloxacin in the presence of excipients from tablets by UPLC-MS/MS and DSC

Parameter CIP MOX NOR OFL
RT (min) a 2.52 ± 0.05 3.22 ± 0.05 2.44 ± 0.05 2.47 ± 0.04
Resolution a,b 1.50 0.92 1.51 0.80
LOD (mg mL -1 ) 0.06 0.06 0.09 0.10
LOQ (mg mL -1 ) 0.18 0.18 0.26 0.30
Linear range (mg mL -1 ) 0.18 – 2.00 0.18 – 1.50 0.26 – 2.00 0.30 – 1.50
Regression equation (y):     
Slope (a ± S a ) 139288.9 ± 1140.1 286408.6 ± 3181.1 166591.5 ± 2043.2 284748.3 ± 5341.4
Intercept (b ± S b ) −635.2 ± 1176.9 4474.6 ± 2691.5 −228.6 ± 2067.2 4043.2 ± 4455.5
t = b/S b 0.54 < tα,f statistically insignificant 1.66 < tα,f statistically insignificant 0.11 < tα,f statistically insignificant 0.91 < tα,f statistically insignificant
Normality of residuals c (Shapiro-Wilk test) 0.9343 (p = 0.49) 0.9619 (p = 0.81) 0.9387 (p = 0.54) 0.9462 (p = 0.62)
Correlation coefficient 0.9997 0.9995 0.9994 0.9986
R 2 value 0.9994 0.9989 0.9986 0.9968
Precision (% RSD) 1.04 0.74 1.09 0.64
Intermediate precision (% RSD) 1.30 0.99 1.18 0.80
  1. Regression equation y = ac + b; c - concentration of solution; y- peak area; Sa – standard deviation of slope;Sb - standard deviation of intercept, t- calculated value of Student’s t-test, tα,f =2.776 critical value of Student’s t-test for degrees of freedom f = 4 and significance level α = 0.05;
  2. aMean ± SD (n = 6).
  3. bResolutions were calculated between two adjacent peaks.
  4. c normal distribution of residuals if p > 0.05.