Skip to main content

Table 1 Influence of the lipase source on the synthesis of D- isoascorbyl palmitate

From: D-isoascorbyl palmitate: lipase-catalyzed synthesis, structural characterization and process optimization using response surface methodology

Lipase Origin Immobilized matrixe Effective temperature (°C) Specific activity Water content Conversion rate (%)a
Novozyme 435 Candida antarctica Macroporous acrylic resin 40-60 10,000PLU/gb 1-2% 41.30 ± 2.6
Lipozyme TLIM Thermomyces lanuginosus Silica granulation 55-70 250IUN/gc 5% 4.30 ± 1.9
Lipozyme RMIM Rhizomucor miehei Anionic exchange resin 30-70 5-6BAUN/gd 2-3% 15.20 ± 3.5
LVK-H100 Aspergillus nige   15-45 20,000U/g   0
LBK-B400 Aspergillus nige   25-65 30,000U/g   0
  1. a: Reaction conditions: D-Isoascorbic 2.5 mmol palmitic acid 10 mmol (Molar ratio was 1:4), lipase load: 15% (weight % of substrates), temperature 50°C, tert-amyl alcohol 20 mL, 50 g/L molecular sieve 4 Å and 200 rpm speed for 24 h.
  2. b: PLU is based on a reaction between propyl alcohol and lauric acid.
  3. c: Interesterification Unit ( IUN) is international unit, based on tributyrin assay.
  4. d: Batch Acidolsis Units Novo (BAUN) is based on a reactionbetween high oleic sunflower oil and decanoic acid at 70- 80°C for 60 min.