Quality assessment on Polygoni Multiflori Caulis using HPLC/UV/MS combined with principle component analysis

BMC Chemistry

Table 3 Regression data, LODs and LOQs for the three analytes tested in HPLC-UV chromatograms

Analyte	Calibration curve^a	R ²	Linear range (mg/L)	LOD^b(ng/mL)	LOQ^c(ng/mL)
1	y = 29885 x - 210960	0.9990	0.404-252.5	98.08	404.03
2	y = 38332 x + 23549	0.9999	0.0443-138.325	44.26	180.56
3	y = 22565 x - 1352.2	0.9997	0.123-15.4	56.77	132.20

1: THSG.
2: emodin.
3: physcion.
^ay is the peak area in UV chromatograms, x is the concentration (mg/L) of the analyte.
^b LOD refers to the limit of detection, s/n = 3.
^c LOQ refers to the limit of quantification, s/n = 10.

ISSN: 2661-801X