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Table 1 pH Optimization of phosphate buffer

From: Stability indicating HPLC method for the simultaneous determination of moxifloxacin and prednisolone in pharmaceutical formulations

Mobile phase Retention Capacity (k/) Theoretical plates (N) Tailing Factor (T) Resolution (R) Peak shape
Methanol: Phosphate Buffer
pH 2.8 (62:38)
Moxifloxacin   4521 1.17   +++
Prednisolone 0.92 6568 1.01 13.66 +++
Methanol: Phosphate Buffer
pH 3.5 (62:38)
Moxifloxacin   4251 1.29   +++
Prednisolone 0.84 5784 1.18 12.24 +++
Methanol: Phosphate Buffer
pH 4.5 (62:38)
Moxifloxacin   4122 1.47   +++
Prednisolone 0.54 5541 1.14 9.45 ---
Methanol: Phosphate Buffer
pH 6.5 (62:38)
Moxifloxacin   3801 1.40   +++
Prednisolone 0.41 4787 1.28 11.87 ---
  1. Chromatographic conditions, mobile phase methanol: 0.018 M phosphate buffer 62:38, pH 2.8, or 3.5, or 4.5, or 6.5, Column BDS Hypersil C8 (250 X 4.6, 5 μm), flow rate 1.5 mL min-1,
  2. injection volume 20 μL, wavelength 254 nm, +++ is peak acceptable and --- is peak not acceptable.