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Table 1 Effect of factor treatment on the mean number of neurons, and the neuron to glial cell ratio in cultures of enteric ganglion cells after 12 and 36 hours incubation

From: Ageing of enteric neurons: oxidative stress, neurotrophic factors and antioxidant enzymes

 

Control

NT-3

GDNF

 

Neurons

Neurons/ glia

Neurons

Neurons/ glia

Neurons

Neurons/ glia

12 hours

159 (±0.36)

0.31

169 (±0.41)

0.36

176 (±0.55)

0.37

36 hours

119 (±0.32)

0.23

151 (±0.37)

0.29

195 (±0.44)

0.34

  1. Cultures established as described in the Methods were grown in the presence of NT-3 or GDNF, both at 10 ng/ml concentration, fixed after 12 or 36 hours and subsequently stained with PGP 9.5. Values represent the mean of neuronal numbers counted in 3 separate experiments, each treatment consisting of 3 replicas per experiment. Values in parentheses represent ± SEM.