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Table 1 Linear regression data and validation parameters of prednisolone acetate (1) and chloramphenicol (2).

From: Stress degradation studies and development of stability-indicating TLC-densitometry method for determination of prednisolone acetate and chloramphenicol in their individual and combined pharmaceutical formulations

Parameters Prednisolone acetate
(at λmax 243 nm)
Chloramphenicol
(at λmax 278 nm)
Linearity range 200-6000 ng/spot 200-6000 ng/spot
Correlation coefficient, r2 ± SD 0.9966 ± 3.5 0.9920 ± 2.5
Slope ± SD 2.42 ± 0.5 2.74 ± 0.34
Intercept ± SD 1106 ± 0.48 4183 ± 2.15
Y = mx + c 2.42x + 1106 2.738x + 4183
Intra-day (n = 3), % RSD 1.32 1.09
Inter-day (n = 3), % RSD 3.90 1.68
Limit of detection 4.77 ng/μL 3.0 ng/μL
Limit of quantification 14.46 ng/μL 9.12 ng/μL
Robustness Robust Robust
Specificity Specific Specific