Table 1 Linear regression data and validation parameters of prednisolone acetate (1) and chloramphenicol (2).
Parameters | Prednisolone acetate (at λmax 243 nm) | Chloramphenicol (at λmax 278 nm) |
|---|---|---|
Linearity range | 200-6000 ng/spot | 200-6000 ng/spot |
Correlation coefficient, r2 ± SD | 0.9966 ± 3.5 | 0.9920 ± 2.5 |
Slope ± SD | 2.42 ± 0.5 | 2.74 ± 0.34 |
Intercept ± SD | 1106 ± 0.48 | 4183 ± 2.15 |
Y = mx + c | 2.42x + 1106 | 2.738x + 4183 |
Intra-day (n = 3), % RSD | 1.32 | 1.09 |
Inter-day (n = 3), % RSD | 3.90 | 1.68 |
Limit of detection | 4.77 ng/μL | 3.0 ng/μL |
Limit of quantification | 14.46 ng/μL | 9.12 ng/μL |
Robustness | Robust | Robust |
Specificity | Specific | Specific |