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Disposable screen-printed sensors for determination of duloxetine hydrochloride

An Erratum to this article was published on 24 July 2012

Abstract

A screen-printed disposable electrode system for the determination of duloxetine hydrochloride (DL) was developed using screen-printing technology. Homemade printing has been characterized and optimized on the basis of effects of the modifier and plasticizers. The fabricated bi-electrode potentiometric strip containing both working and reference electrodes was used as duloxetine hydrochloride sensor. The proposed sensors worked satisfactorily in the concentration range from 1.0 × 10-6-1.0 × 10-2 mol L-1 with detection limit reaching 5.0 × 10-7 mol L-1 and adequate shelf life of 6 months. The method is accurate, precise and economical. The proposed method has been applied successfully for the analysis of the drug in pure and in its dosage forms. In this method, there is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated statistically by recovery studies.

1. Introduction

Duloxetine hydrochloride (DL), chemically known as (+) - (S) - N-methyl - γ - (1- naphthyloxy) -2-thiophenepropylamine hydrochloride [1], is an antidepressant agent. It is indicated for the treatment of major depressive disorder (MDD) and general anxiety disorder. It is a selective serotonin and nor-epinephrine reuptake inhibitor (SSNRI) for oral administration. It is also used to treat fibromyalgia (a chronic pain disorder), or chronic muscle or joint pain (such as low back pain and osteoarthritis pain). It may also used to treat pain caused by nerve damage in people with diabetes [2]. DL is considered among the most important antidepressant drugs therefore, the development of reliable rapid and accurate procedures for this active ingredient quantification is welcomed [3]. A survey of literature showed that several analytical methods have been reported for determination of DL in its pure form, pharmaceutical preparations and biological fluids, where DL is not determined officially in any pharmacopoeia. These methods include high-performance liquid chromatography [4, 5], liquid chromatography coupled with mass spectrometry [68], high performance thin layer chromatography [9], Spectrofluorimetry [10], spectrophotometry [11] and capillary zone electrophoresis [12]. Although, those methods are very sensitive, they are not adapted for in situ and real time detection of DL as they are time consuming, involve expensive apparatus and require skilled technicians.

Electrochemical techniques are of choice since they possess the advantages of simplicity, accuracy, and low cost without separation or pretreatment procedures [13]. The conventional Polyvinylchloride (PVC) membrane ion-selective electrodes have found a wide range of applications in pharmaceutical analysis; however they still have certain inherent limitations. Drawbacks in the use of PVC electrodes were arisen from the time consuming and inconsistent manual fabrication typically employed as well as short lifetime of these electrodes [14].

To overcome the aforementioned difficulties in PVC membrane electrodes, new kinds of all solid- state ones (without internal reference solution) allowing electrode miniaturization capability were introduced. Coated wire electrodes (CWEs) and coated graphite electrodes are examples of these sensors design; however the potential drift and the poor adhesion of the membrane to the metal substrate are main drawbacks of these electrodes [15]. Carbon paste electrodes (CPEs) had been employed as useful materials for the fabrication of simple sensors since 1970s [16]. In comparison to PVC electrodes the CPEs possessed advantages of much lower Ohmic resistance, very fast and stable response with easy renewal of the electrode surface as well as long functional lifetime. As CPEs continued to play a major role in the development of analytical procedures or testing new analytical methodology, screen-printed electrodes (SPEs) could be considered a solution for the lack of commercial availability of CPEs. Screen printing seems to be one of the most promising technologies providing high versatility, reproducibility, and large-scale production for commercialization [17].

The present work aimed to introduce disposable screen-printed carbon electrodes (SPCEs) as potentiometric sensors using homemade carbon ink. SPCEs were fabricated in plain and modified forms, and then subjected to a series of tests to select the sensor possessing the most favorable analytical characteristics for their application as potentiometric sensor for DL determination in bulk drug and in its pharmaceutical formulations.

2. Experimental

2.1. Reagents

All reagents were of analytical grade and distilled water was used throughout the experiments. Di-butyl phthalate (DBP), di-octyl phthalate (DOP), di-octyl sebacate (DOS) and di-butyl sebacate (DBS), (Sigma-Aldrich) were used as electrode plasticizers. Poly (vinyl chloride) (PVC), with relative high molecular weight, phosphomolybdic acid (PMA), phosphotungstic acid (PTA), ammonium reineckate salt (ARS), acetone, cyclohexanone and tetrahydrofuran (THF) were from (Sigma-Aldrich, Germany) and graphite powder, synthetic 1-2 μm, (Dorset, UK) were applied in electrode fabrication. Duloxetine hydrochloride was kindly supplied from Jean Radne Sales (Indian). Cymbalta® 60 mg/capsule was purchased from local drug stores.

2.2. Apparatus

Potential measurements were carried out using HANNA pH meter 211 with a combined pH glass electrode. Scanning electron microscope (JEOL JSM-6060 LV-Japan) was used for surface structure studies.

2.3. Authentic samples

Stock drug solution 0.1 mol L-1 was prepared by dissolving 1.7 g of the drug in distilled water, filtered and completed to 50 mL with distilled water.

2.4. Pharmaceutical preparation

The content of ten capsules of Cymbalta® 60 mg/capsule was weighed, ground, and an accurate weight of the powder assigned to contain 100 mg DL was dissolved in distilled water, filtered and completed to 50 mL with distilled water.

2.5. Composition of screen printing carbon electrodes

SPCEs are heterogeneous carbon electrodes having a composite structure since the printing ink matrix colligates different phases (carbon powder, plasticizer and polymeric binder which is PVC dissolved in a proper organic solvent). After printing and curing, carbon tracks were deposited on the substrate with a final composition of 34.6% plasticizer, 57.7% carbon and 7.7% polymeric binder. This composition combines those of both carbon pastes (28.6% plasticizer and 71.4% carbon) and PVC membranes (50% plasticizer, and 50% PVC). Scanning electron microscopy (SEM) indicated that addition of the plasticizer into the printing carbon ink produced a semi-liquid phase with smooth electrode surface (Figure 1). Such unique composition makes the SPCEs to combine the electroanalytical behavior of both PVC and CPE.

Figure 1
figure 1

Scanning electron micrographs of electrode surface. a) Carbon ink containing DOP, b) Carbon ink without DOP.

2.6. Optimization of electrode performance

2.6.1. Plain or soaked electrodes

Homemade carbon ink was prepared by mixing of 0.8 g carbon powder, 0.5 g plasticizer and 1.3 g 8% PVC binder dissolved in a proper organic solvent 5 mL (THF). It is expected that during soaking of such electrode in the aqueous DL-ion pair(s) suspension, the electrode plasticizer extracts these ion-pair(s) and the electrode bulk becomes gradually saturated with such ion-pair(s), and hence there is no need to incorporate the ion exchanger into the electrode during its preparation. The plain SPCEs were soaked in different DL-ion pair(s) suspensions and used in potentiometric determination of DL hydrochloride [18].

2.6.2. Modified electrode with duloxetine- ion pairs

DL forms water-insoluble ion pair complexes with 20 mg for each ammonium reineckate salt (ARS), phosphotungstic acid (PTA) and phosphomolybdic acid (PMA) which can be used as electoactive materials for DL sensors.

2.6.3. Modified electrode with the ion pairing agents (in situ mode)

In the modified electrode with ion pairing agents a suitable ion pairing agent PMA, 10 to 50 mg was used in the printing ink followed by soaking of printed electrodes in DL solution led to the formation of DL-ion pairs at electrode surface which were subsequently extracted by the electrode mediator (plasticizer) into the electrode bulk solution.

2.6.4. Effect of plasticizer type

In this study the effect of plasticizer type on the performance of screen printing carbon electrodes has been studied. Four plasticizers, dioctylphthalate (DOP), dibutylphthalate (DBP), dioctylsebacate (DOS) and diobutylsebacte (DBS) were used to examine the optimization of electrode performance. The content ratio were: for plasticizer 34.6, 45.0, 40.5 and 36.7w%, for Carbon powder 57.7, 45.0, 54.8 and 55.5w% and for PVC 7.7, 10.0, 8.5 and 4.5w%.

2.6.5. Sensors construction

The potentiometric strips were fabricated in arrays of six couples consisting of the working and reference electrodes (each 5 × 35 mm) following the procedures described elsewhere [17]. Ag/AgCl pseudo-reference electrode was first printed using homemade ink (prepared by mixing 0.9 g Ag/AgCl mixture (65:35%) with 0.8 g of 8% PVC solution in acetone-cyclohexanone mixture) and cured at 60°C for 30 min. The plain carbon electrode was printed using carbon ink (prepared by mixing 0.5 g DOP, 1.3 g of 8% PVC solution and 0.8 g carbon powder), cured at 50°C for 30 min and soaked in freshly prepared DL-ion pair suspensions for 24 h. The modified electrodes were fabricated in the same manner with incorporation of either 20 mg of phosphomolybdic acid ion pair or 50 mg ammonium reineckate ion pair as senescing materials and used directly in the potentiometric measurements after 10 min preconditioning in 1.0 × 10-3 mol L-1 DL solution.

2.6.6. Potentiometric measurements

The fabricated sensors were calibrated by immersing the bi-electrode strip in 5 mL aliquots of 1.0 × 10-6-1.0 × 10-1 mol L-1 DL solutions and the potential readings were plotted against drug concentration (-log [DL]).

2.6.7. Analytical applications

Using standard addition method, an electrode was immersed into a sample of 5 mL with unknown concentration (ca. 1.0 × 10-4 mol L-1 and the equilibrium potential of E1 was recorded. Then 0.1 mL of 1.0 × 10-1 mol L-1 standard solution of DL was added into the testing solution and the equilibrium potential of E2 was obtained. From the change of Δ (E 2 -E 1 ) one can determine the concentration of the testing sample [19].

In the potentiometric titration methods, aliquots of the sample solutions containing 1.0 × 10-6-1.0 × 10-2 mol L-1 DL were titrated with standard phosphomolybdic acid solution and the electrode potential values were plotted against volume of the titrant added to estimate the end point.

3. Results and discussion

Both unmodified and modified electrodes (either with DL ion pairs or ion pairing agents) were prepared and tested for the effect of nature and content of modifier, type of plasticizer, pH, response time, interferents, and applications.

3.1. Response characteristics of DL-SPCEs sensors

It is expected that during soaking of the plain electrode in the aqueous DL-ion pair(s) suspension, the electrode plasticizer extracts these ion-pair(s) and the electrode bulk becomes gradually saturated with such ion-pair(s), and hence there is no need to incorporate the ion exchanger into the electrode during its preparation. The plain SPCEs were soaked in different DL-ion pair(s) suspensions and used in potentiometric determination of DL hydrochloride. Results obtained showed that the electrodes soaked in DL-phosphomolybdate gave the best performance indicated by the highest slope (57 ± 0.4 mV decade-1) which may be attributed to the difference in solubility products of these ion pairs and their extraction into the electrode bulk solution [20], (Table 1).

Table 1 Optimization of composition (w/w %) of SPCEs sensors

DL forms water-insoluble ion pair complexes with ammonium reineckate salt (ARS), phosphotungstic acid (PTA) and phosphomolybdic acid (PMA) which can be used as electoactive materials for DL sensors. SPCEs incorporated with different DL ion-pairs gave Nernstain responses with different slopes and sensitivities depending on the nature of the ion pair used. Incorporation of DL-PMA showed the best performance (slope 55 ± 2 mV decade-1) compared to the other DL-ion pairs modified electrodes. The content of DL-PMA in the printing ink was varied from 5 to 50 mg; incorporation of 20 mg was found to be the best (58 ± 0.8 mV decade-1) as further addition of this ion pair resulted in decreasing of the electrode slope as it reaches 47 mV decade-1.

Incorporation of a suitable ion pairing agent in the printing ink followed by soaking of printed electrodes in DL solution led to the formation of DL-ion pairs at electrode surface which were subsequently extracted by the electrode mediator (plasticizer) into the electrode bulk solution. From different incorporated ion-pairing agents, phosphomolybdic acid showed superior sensitivity indicated by the highest slope value (54 ± 2 mV decade-1) compared to the other ion pairing agents. Carbon inks containing 10 to 50 mg phosphomolybdic acid were prepared and used in printing of electrodes and calibration results revealed that SPCEs containing 50 mg of phosphomolybdic acid showed the highest slope (55 ± 2 mV decade-1).

3.2. Plasticizer effect

The influence of the plasticizer on the electrode performance has been studied by comparing the DOP plasticized electrode with those plasticized with DBP, DOS and DBS Figure 2. The obtained calibration graphs of plain SPCEs clarified that, using DOP as plasticizer offers better sensitivity indicated by the highest slope (slope values were 58 ± 0.7, 53 ± 0.9, 54 ± 1 and 55 ± 0.8 mV decade-1 for DOP, DBP, DOS and DBS, respectively) which may be related to the dielectric constant of plasticizers and their abilities to extract DL-PMA into the electrode matrices (ε = 17.6, 3.8, 4.5 and 5.2 for tested plasticizers, respectively).

Figure 2
figure 2

Effect of pH using 1.0 × 10-3 mol L-1 DL on the potential of DL sensors.

3.3. Sensors performance

The potentiometric response characteristics of DL-SPCEs were evaluated according to IUPAC recommendations [21]. Sensors displayed (Table 2) linear response from 1.0 × 10-6 to 1.0 × 10-2 mol L-1 DL with Nernstian cationic slopes depending on the mode of preparation. Both SPCEs modified with the DL-PMA and plain electrodes showed better performance relative to those incorporated with the ion pairing agent indicated by higher slopes (58 ± 0.8 and 57 ± 0.4 mV decade-1) with lower detection limit reaching 5.0 × 10-7 mol L-1. Screen-printing technology offers production reproducibility, versatility, and large-scale production for commercialization. Concerning the fabrication reproducibility, modification with DL-PMA was the best as the average slope values for six electrodes within the same batch were 58 ± 0.8 mV decade-1 with standard potential 37 ± 2 mV. The corresponding values between three different batches were 59 ± 1 mV decade-1 and 375 ± 2.7 mV, respectively. The lifetimes of the different fabricated electrodes were tested by performing day-to-day calibration. SPCEs showed a useful lifetime of 4 weeks of daily measurement, during which the average slopes were reproducible (within ± 2 mV decade-1). Moreover, the SPCEs showed shelf life of more than 6 months when stored dry at 4°C without significant change in the electrode performance. The dynamic response time of the electrode was tested by measuring the time required to achieve a steady state potential (within ± 1 mV) after sudden increase in the DL concentration from 1.0 × 10-6 to 1.0 × 10-2 mol L-1. The response time of both plain and DL-PMA modified SPCEs was fast (as about 2.8 s); while electrodes modified with the ion pairing agents gave a stable potential reading after 5 s. The influence of pH on the response of SPCEs was also checked by recording the potential readings of the cell in solutions containing 1.0 × 10-4 to 1.0 × 10-2 mol L-1 DL at different pH values (pH 2-9). The plots of E (mV) versus pH indicated that the electrodes responses were found to be pH-independent in the range 3-8 (Figure 2). Selectivity coefficients of DL-SPCEs were determined using separate solution method [19] and matched solution method [22]. Additives and fillers commonly introduced in pharmaceutical formulations (such as glycine, caffeine, citrate, maltose, sucrose, and starch) as well as inorganic cations, Na+, K+, Li+, Ca2+, Mg2+, and NH4+ did not show significant interference in the determination of DL (Table 3).

Table 2 Analytical performance of various DL-SPCEs
Table 3 Selectivity and tolerance values for the DL-PMA responsive sensors

3.4. Potentiometric titration

In addition to the application of SPCEs in direct potentiometric determination of DL hydrochloride, the developed sensors were also used as end-point indicator electrodes in the potentiometric titration of DL hydrochloride with phosphomolybdic acid. The effect of electrode plasticizer on the titration performance was also investigated. Generally, the printed electrodes plasticized with DOP gave the highest total potential change (ΔE = 380 mV) compared with those plasticized with DBS, DOS and DBP (ΔE = 180, 148 and 90 mV for the plasticizers in the same order). Moreover, plain SPCEs showed the best titration curve (Figure 3) comparable to those modified with either the DL-PMA or ion-pairing agent. Under the same conditions, SPCEs showed a better titration curve than that obtained with CPEs and similar to PVC electrode (Figure 4). The titration curves were symmetrical with very well-defined inflexion points of potential jumps indicating the high sensitivity of the electrode. The titration process was highly reproducible with average recoveries 99.6 ± 0.8 and 99.6 ± 0.6% for 1.0 × 10-2 and 1.0 × 10-3 mol L-1 DL, respectively. It was found that the same electrode was successfully applied for more than 20 titration processes over 20 days with non-significant drift in the total potential change (ΔE = 286 ± 1 mV) and average recovery of 99.98 ± 0.8%.

Figure 3
figure 3

Potentiometric titration of DL with phosphomolybdic acid 5.0 × 10-3 mol L-1 using SPCEs fabricated with different modes (2.0 mL of 1.0 × 10-2 mol L-1 DL).

Figure 4
figure 4

Potentiometric titration of DL with phosphomolybdic acid 5.0 × 10-3 mol L-1 using SPCEs fabricated with different modes SPCE, PVC and CPEs (1.0 mL of 1.0 × 10-2 mol L-1 DL).

3.5. Analytical applications

The proposed electrodes were successfully employed for assay of DL in its authentic samples as well as pharmaceutical formulations applying standard addition, and potentiometric titration methods. The results in Table 4 clearly indicate satisfactory agreement between the DL contents in different samples determined by the developed sensor and the reported UV-spectrophotometric method [23] which includes the determination of duloxetine hydrochloride in 0.1 mol L-1 HCl at 288 nm.

Table 4 Potentiometric determination of DL in pharmaceutical preparation using SPCEs

4. Conclusion

The present study shows that, the SPCEs can be characterized, optimized, and successfully applied for the potentiometric determination of DL using the procedures adapted in the application of the ordinary CPEs and PVC electrodes. SPCEs are produced more easily and rapidly, saving large amounts of reagents, especially sensing material. The developed disposable strips have several advantages including simplicity, versatility, reproducibility of the preparation, and low cost mass production. Moreover, the different DL sensors presented in this study showed fast dynamic response times and longer lifetimes. The electrochemical performance of the developed SPCEs was even better than the conventional ones and can be regarded as the base for further research on drug quality control via incorporation of different sensing materials.

References

  1. Sweetman SC: Martindale: The complete drug reference. 2005, London: Pharmaceutical Press, 292-34

    Google Scholar 

  2. Brunton LL, Parker KS, Lazo JS: Goodman and Gillman's, the Pharmacological Basis of Therapeutics. 2005, London: McGraw Hill Publishing, 436-11

    Google Scholar 

  3. Aboul-Enein HY, Stefan RI: The opportunity to use ion-selective membrane electrodes for dissolution tests. Inst Sci &Tech. 1999, 27 (2): 89-93.

    Article  CAS  Google Scholar 

  4. Raman NV, Harikrishna KA, Prasad AV, Reddy KR, Ramakrishna K: Determination of duloxetine hydrochloride in the presence of process and degradation impurities by a validated stability-indicating RP-LC method. J Pharm Biomed Anal. 2010, 51 (4): 994-997. 10.1016/j.jpba.2009.10.025.

    Article  CAS  Google Scholar 

  5. Sinha VR, Anamika , Kumria R, Bhinge JR: Stress degradation studies on duloxetine hydrochloride and development of an RP-HPLC method for its determination in capsule formulation. J Chromatogr Sci. 2009, 47 (7): 589-593.

    Article  CAS  Google Scholar 

  6. Senthamil Selvan P, Gowda KV, Mandal U, Sam Solomon WD, Pal TK: Determination of duloxetine in human plasma by liquid chromatography with atmospheric pressure ionization-tandem mass spectrometry and its application to pharmacokinetic study. J Chromatogr B. 2007, 858 (1-2): 269-275. 10.1016/j.jchromb.2007.09.002.

    Article  CAS  Google Scholar 

  7. Ma N, Zhang BK, Li HD, Chen BM, Xu P, Wang F, Zhu RH, Feng S, Xiang DX, Zhu YG: Determination of duloxetine in human plasma via LC/MS and subsequent application to a pharmacokinetic study in healthy Chinese volunteers. Clin Chim Acta. 2007, 380 (1-2): 100-105. 10.1016/j.cca.2007.01.018.

    Article  CAS  Google Scholar 

  8. Satonin DK, McCulloch JD, Kuo F, Knadler MP: Development and validation of a liquid chromatography-tandem mass spectrometric method for the determination of the major metabolities of duloxetine in human plasma. J Chromatogr B. 2007, 852 (1-2): 582-589. 10.1016/j.jchromb.2007.02.025.

    Article  CAS  Google Scholar 

  9. Dhaneshwar SS, Deshpande P, Patil M, Vadnerkar G, Dhaneshwar SR: Development and validation of HPTLC method for estimation of duloxetine hydrochloride in bulk drug and in tablets dosage form. Indian J Pharm Sci. 2008, 70 (2): 233-236. 10.4103/0250-474X.41463.

    Article  Google Scholar 

  10. Prabu SL, Shahnawaz S, Kumar CD, Shirwaikar A: Spectrofluorometric method for determination of duloxetine hydrochloride in bulk and pharmaceutical dosage forms. Indian J Pharm Sci. 2008, 70 (4): 502-503. 10.4103/0250-474X.44603.

    Article  CAS  Google Scholar 

  11. Kamila MM, Mondal N, Ghosh LK: A validated UV spectrophotometric method for determination of duloxetine hydrochloride. Die Pharmazie. 2007, 62 (6): 414-415.

    CAS  Google Scholar 

  12. Musenga A, Amore M, Mandrioli R, Kenndler E, de Martino L, Raggi MA: Determination of duloxetine in human plasma by capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr B. 2009, 877 (11-12): 1126-1132. 10.1016/j.jchromb.2009.02.059.

    Article  CAS  Google Scholar 

  13. Cosofret VV, Buck RP: Pharmaceutical Applications of membrane sensors. 1992, CRC Press; Boca Raton, FL

    Google Scholar 

  14. Abbas MN, Radwan AA: Novel lipoate-selective membrane sensor for the flow injection determination of α-lipoic acid in pharmaceutical preparations and urine. Talanta. 2008, 74: 1113-1121. 10.1016/j.talanta.2007.08.008.

    Article  CAS  Google Scholar 

  15. Singh AK, Singh P: Nano-level monitoring of Yb(III) by fabrication of coated graphite electrode based on newly synthesized hexaaza macrocyclic ligand. Anal Chim Acta. 2009, 643 (1-2): 74-82. 10.1016/j.aca.2009.03.047.

    Article  CAS  Google Scholar 

  16. Mesaric S, Dahmen EAMF: Ion selective carbon-paste electrodes for halides and silver (I) ions. Anal Chim Acta. 1973, 64 (3): 431-438. 10.1016/S0003-2670(01)82475-2.

    Article  CAS  Google Scholar 

  17. Khaled E, Hassan HN, Girgis A, Metelka R: Construction of novel simple phosphate screen-printed and carbon paste ion-selective electrodes. Talanta. 2008, 77: 737-743. 10.1016/j.talanta.2008.07.018.

    Article  CAS  Google Scholar 

  18. Elmorsy K, Hasan HNA, Gehad GM, Seleim AA: Ion selective electrode for the determination of trazodone in tablets. Talanta. 2010, 81 (1-2): 510-515. 10.1016/j.talanta.2009.12.033.

    Article  Google Scholar 

  19. Ma TS, Hassan SSM: Organic analysis using ion selective electrode. 1982, Academic Press, London, I and II:

    Google Scholar 

  20. Khaled E, Hassan HN, Mohamed GG, Seleim AE: Towards disposable sensors for drug quality control: Dextromethorphan screen-printed electrodes. Drug Test Anal. 2010, 2: 424-429. 10.1002/dta.159.

    Article  CAS  Google Scholar 

  21. Umezawa Y, Buhlmann P, Umezawa K, Tohda K, Amemiya S: Potentiometric selectivity coefficients of ion-selective electrodes, part I, inorganic cations (IUPAC Technical Report). Pure Appl Chem. 2000, 72: 1851-2082. 10.1351/pac200072101851.

    Article  CAS  Google Scholar 

  22. Buck RP, Lindner E: Recommendations for nomenclature of ion-selective electrodes. Pure Appl Chem. 1994, 66: 2527-2536. 10.1351/pac199466122527.

    Article  CAS  Google Scholar 

  23. Yunoos M, Sankar DG, Kumar BP, Hameed S, Hussain A: Simple UV spectrophotometric determination of duloxetine hydrochloride in bulk and in pharmaceutical formulations. E-journal of Chem. 2010, 7 (3): 785-788.

    Article  CAS  Google Scholar 

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7. Acknowledgments

The Authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project No. RGP-VPP-062.

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Correspondence to Maha F El-Tohamy.

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5. Competing interests

The authors declare that they have no competing interests.

6. Authors' contributions

NAA participated the design of the study and performed the statistical analysis of the data, RAA provided the chemicals and pure materials, conceived of the study, participated in its design and coordination and MFE carried out the practical studies and write the manuscript. All The authors read and approved the manuscript.

A retraction note to this article can be found online at http://dx.doi.org/10.1186/1752-153X-6-72.

An erratum to this article is available at http://dx.doi.org/10.1186/1752-153X-6-72.

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Alarfaj, N.A., Ammar, R.A. & El-Tohamy, M.F. Disposable screen-printed sensors for determination of duloxetine hydrochloride. Chemistry Central Journal 6, 6 (2012). https://doi.org/10.1186/1752-153X-6-6

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