Figure 8
HCT116 cell images were taken under an invented phased-contrast microscope at ×200 magnification with a digital camera at 48 hours after treatment with the samples. A) Cells from the control group showed fully confluent growth. B) Treatment with 8.2PF6 showed marked inhibition in cell proliferation with IC50=0.2μM. The picture revealed the autophagic characteristic in the cells as all cells treated with 8.2PF6 showed abnormally large number of vacuoles (arrows) in cytoplasm. C) HCT 116 cells treated with the compound 9.2PF6 exhibited significant cytotoxity (IC50=4.1μM) as the population of cells reduced drastically within the 48 hours of treatment. D) Photomicrograph depicts the strong cytotoxic effect of 10.2Br (0.1μM). It can be seen clearly that the compound affected the normal morphology of most all the cells of the group which rendered the cells lose viability. E) Treatment with 11.2PF6 showed the modest inhibition with IC508.7μM. F) Treatment with 12.PF6 caused sever death in HCT 116 cells as the cells appeared to be entered in apoptosis by the typhical morphological changes.