Effective separation and simultaneous analysis of anabolic androgenic steroids (AAS) in their pharmaceutical formulations by a validated TLC-densitometry method

Table 1 R _f values and peak widths of the compounds in different mobile phases

S.No	Solvent composition	Ratios (v/v)	Retention factor value (R_f)				ΔR_f1	ΔR_f2	ΔR_f3	Peak Widths (cm)
S.No	Solvent composition	Ratios (v/v)	TPP	TP	TI	TD	ΔR_f1	ΔR_f2	ΔR_f3	TPP	TP	TI	TD
1	Toluene-Ethyl acetate	9.6:0.4	0.26	0.3	0.31	0.36	0.04	0.01	0.05	0.04	0.02	0.04	0.05
2	Toluene-Ethyl acetate-chloroform	9.0:0.6:0.4	0.35	0.41	X	0.46	0.06	X	0.05	0.05	0.06	X	0.05
3	Hexane-chloroform-ethyl acetate	7.0:1.0:1.5	0.63	X	0.67	0.73	X	0.04	0.06	0.09	X	0.06	0.05
4	Hexane-chloroform-ethyl acetate	7.5:1.5:1.0	0.23	X	0.29	0.33	X	0.06	0.04	0.08	X	0.04	0.05
5	Hexane-chloroform-ethyl acetate	8.3:1.0:0.7	0.36	0.38	0.44	0.48	0.02	0.06	0.04	0.04	0.04	0.05	0.06
6	Hexane-chloroform-ethyl acetate	8.3:0.7:1.0	0.38	0.41	0.46	0.51	0.03	0.05	0.05	0.04	0.04	0.06	0.05
7	Hexane-dichloromethane-ethyl acetate	8.0:0.7:1.3	0.54	0.54	0.61	0.65	X	0.07	0.04	0.1	X	0.05	0.06
8	Hexane-dichloromethane-ethyl acetate	7.5:1.0:1.5	0.67	X	0.74	0.77	X	0.07	0.03	0.11	X	0.05	0.05
10	Hexane-Ethyl acetate (Macherey-Nagel)	8.5:1.5	0.40	0.43	0.48	0.52	0.03	0.05	0.04	0.04	0.04	0.04	0.04
11	Composition 10 (TLC Plates-Merck)	8.5:1.5	0.31	0.34	0.40	0.45	0.03	0.06	0.05	0.05	0.04	0.06	0.05
12	Composition 10 (HPTLC Plate)	8.5:1.5	0.29	0.31	0.35	0.37	0.02	0.04	0.02	0.04	0.03	0.03	0.04

X = Shows no separate peak detected, Δ R_{f1 =} between TPP and TP, Δ R_{f2 =} between TP and TI, Δ R_f3= between TI and TD.

ISSN: 2661-801X