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Table 1 R f values and peak widths of the compounds in different mobile phases

From: Effective separation and simultaneous analysis of anabolic androgenic steroids (AAS) in their pharmaceutical formulations by a validated TLC-densitometry method

S.No Solvent composition Ratios (v/v) Retention factor value (Rf) ΔRf1 ΔRf2 ΔRf3 Peak Widths (cm)
TPP TP TI TD TPP TP TI TD
1 Toluene-Ethyl acetate 9.6:0.4 0.26 0.3 0.31 0.36 0.04 0.01 0.05 0.04 0.02 0.04 0.05
2 Toluene-Ethyl acetate-chloroform 9.0:0.6:0.4 0.35 0.41 X 0.46 0.06 X 0.05 0.05 0.06 X 0.05
3 Hexane-chloroform-ethyl acetate 7.0:1.0:1.5 0.63 X 0.67 0.73 X 0.04 0.06 0.09 X 0.06 0.05
4 Hexane-chloroform-ethyl acetate 7.5:1.5:1.0 0.23 X 0.29 0.33 X 0.06 0.04 0.08 X 0.04 0.05
5 Hexane-chloroform-ethyl acetate 8.3:1.0:0.7 0.36 0.38 0.44 0.48 0.02 0.06 0.04 0.04 0.04 0.05 0.06
6 Hexane-chloroform-ethyl acetate 8.3:0.7:1.0 0.38 0.41 0.46 0.51 0.03 0.05 0.05 0.04 0.04 0.06 0.05
7 Hexane-dichloromethane-ethyl acetate 8.0:0.7:1.3 0.54 0.54 0.61 0.65 X 0.07 0.04 0.1 X 0.05 0.06
8 Hexane-dichloromethane-ethyl acetate 7.5:1.0:1.5 0.67 X 0.74 0.77 X 0.07 0.03 0.11 X 0.05 0.05
10 Hexane-Ethyl acetate (Macherey-Nagel) 8.5:1.5 0.40 0.43 0.48 0.52 0.03 0.05 0.04 0.04 0.04 0.04 0.04
11 Composition 10 (TLC Plates-Merck) 8.5:1.5 0.31 0.34 0.40 0.45 0.03 0.06 0.05 0.05 0.04 0.06 0.05
12 Composition 10 (HPTLC Plate) 8.5:1.5 0.29 0.31 0.35 0.37 0.02 0.04 0.02 0.04 0.03 0.03 0.04
  1. X = Shows no separate peak detected, Δ R f1 = between TPP and TP, Δ R f2 = between TP and TI, Δ R f3= between TI and TD.