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Table 2 Cytotoxicity activity of the synthesized individual compounds 1-11(A-K)

From: Schiff bases of indoline-2,3-dione (isatin) with potential antiproliferative activity

  Compound/IC50 (μM)
Compound A1 B1 C1 D1 E1 F1 G1 H1 I1 J1 K1
K562 cell line 20.04 ± 0.07 42.3 ± 0.03 13.47 ± 0.038 11.5 ± 0.01 6 ± 0.007 7.7 ± 0.02 5.66 ± 0.11 15.37 ± 0.02 11.59 ± 0.034 > 100 > 100
MCF-12A cell line 69.23 ± 0.123 49.39 ± 0.16 > 300 > 300 > 300 64.31 ± 0.026 16.61 ± 0.181 37.12 ± 0.27 > 300 > 300 > 300
SI 3.45 1.17 > 22.27 > 26.1 > 50.00 8.4 2.9 2.42 > 25.88 NA NA
Compound A2 B2 C2 D2 E2 F2 G2 H2 I2 J2 K2
K562 cell line 49.13 ± 0.095 20.19 ± 0.09 29.82 ± 0.139 8.3 ± 0.003 7.76 ± 0.07 12.9 ± 0.002 5.72 ± 0.1 8.05 ± 0.035 8.67 ± 0.027 23.32 ± 0.32 > 100
MCF-12A cell line 32.55 ± 0.073 > 300 > 300 14.78 ± 0.4 > 300 31.39 ± 0.096 16.62 ± 0.182 11.67 ± 0.003 > 300 17.91 ± 0.62 > 300
SI 0.66 > 14.86 > 10.06 1.8 > 38.66 2.4 2.9 1.45 > 34.60 0.77 NA
Compound A3 B3 C3 D3 E3 F3 G3 H3 I3 J3 K3
K562 cell line 32.37 ± 0.074 19.35 ± 0.019 14.86 ± 0.055 13.86 ± 0.01 7.13 ± 0.009 14 ± 0.08 9.88 ± 0.41 > 100 15.81 ± 0.11 19.75 ± 0.08 > 100
MCF-12A cell line 27.06 ± 0.272 > 300 > 300 258.09 ± 0.03 > 300 8.71 ± 0.17 13.22 ± 0.034 97.36 ± 0.062 27.87 ± 0.07 > 300 83.8± 0.039
SI 0.84 > 15.50 > 20.19 18.6 > 42.08 0.62 1.3 NA 1.76 > 15.19 NA
Compound A4 B4 C4 D4 E4 F4 G4 H4 I4 J4 K4
K562 cell line 38.88 ± 0.138 51.51 ± 0.09 30.96 ± 0.2 9.98 ± 0.009 7.5 ± 0.03 16.28 ± 0.015 8.43 ± 0.163 24.21 ± 0.023 11.57 ± 0.051 14.52 ± 0.08 > 100
MCF-12A cell line 9.39 ± 0.152 > 300 > 300 > 300 > 300 50.85 ± 0.048 12.50 ± 0.216 > 300 > 300 12.74 ± 0.32 78.29 ± 0.31
SI 0.24 > 5.82 > 9.69 > 30 > 40.00 3.1 1.5 > 12.39 > 25.93 0.88 NA
Compound A5 B5 C5 D5 E5 F5 G5 H5 I5 J5 K5
K562 cell line > 100 7.3 ± 0.01 23.9 ± 0.26 8.67 ± 0.007 10.3 ± 0.001 10.4 ± 0.044 6.5 ± 0.001 5.88 ± 0.004 24.11 ± 0.255 6.99 ± 0.005 > 100
MCF-12A cell line 39.74 ± 0.073 > 300 > 300 80.05 ± 0.40 > 300 39.09 ± 0.24 9.1 ± 0.003 7.35 ± 0.094 > 300 5.6 ± 0.003 > 300
SI NA > 41.10 > 12.55 9.2 > 29.13 3.8 1.4 1.25 > 12.44 0.80 NA
Compound A6 B6 C6 D6 E6 F6 G6 H6 I6 J6 K6
K562 cell line > 100 > 100 > 100 8.87 ± 0.017 9.58 ± 0.03 13.5 ± 0.03 6.62 ± 0.22 6.49 ± 0.017 8.55 ± 0.06 7.19 ± 0.051 > 100
MCF-12A cell line 13.91 ± 0.003 > 300 > 300 134.18 ± 0.142 > 300 9.6 ± 0.15 13.05 ± 0.20 34.77 ± 0.062 26.15 ± 0.14 10.16 ± 1.2 > 300
SI NA NA NA 15.1 > 31.32 0.71 2 5.36 3.06 1.41 NA
Compound A7 B7 C7 D7 E7 F7 G7 H7 I7 J7 K7
K562 cell line > 100 47.25 ± 0.027 > 100 9.1 ± 0.026 8.9 ± 0.02 12.86 ± 0.01 7.68 ± 0.34 23.67 ± 0.39 > 100 18.02 ± 0.13 > 100
MCF-12A cell line 11.03 ± 0.151 > 300 > 300 > 300 > 300 11.39 ± 0.25 12.98 ± 0.439 73.18 ± 0.072 > 300 > 300 61.34 ± 0.01
SI NA > 6.35 NA > 33 > 33.71 0.89 1.7 3.09 NA > 16.65 NA
Compound A8 B8 C8 D8 E8 F8 G8 H8 I8 J8 K8
K562 cell line > 100 27.23 ± 0.092 > 100 6.9 ± 0.001 10.34 ± 0.015 6.99 ± 0.007 15.12 ± 0.48 40.65 ± 0.24 > 100 45.51 ± 0.46 > 100
MCF-12A cell line 10.54 ± 0.157 > 300 > 300 > 300 > 300 21.29 ± 0.104 > 300 > 300 > 300 > 300 > 300
SI NA > 11.02 NA > 43.5 > 29.01 3 > 19.8 > 7.38 NA > 6.59 NA
Compound A9 B9 C9 D9 E9 F9 G9 H9 I9 J9 K9
K562 cell line 90.67 ± 0.19 17.42 ± 0.05 > 100 6.2 ± 0.01 9.77 ± 0.01 6.77 ± 0.01 > 100 26.72 ± 0.38 31.19 ± 0.004 52.05 ± 0.15 > 100
MCF-12A cell line 14.48 ± 0.647 > 300 > 300 > 300 > 300 13.14 ± 0.114 > 300 > 300 > 300 > 300 5.88 ± 0.11
SI 0.16 > 17.22 NA > 48.4 > 30.71 1.9 NA > 11.23 > 9.62 > 5.76 NA
Compound A10 B10 C10 D10 E10 F10 G10 H10 I10 J10 K10
K562 cell line > 100 17.88 ± 0.062 > 100 8.9 ± 0.43 8.66 ± 0.06 6.3 ± 0.005 8.43 ± 0.18 23.34 ± 0.19 19.14 ± 0.07 > 100 88.85 ± 0.26
MCF-12A cell line 8.33 ± 0.044 > 300 > 300 12.57 ± 0.089 > 300 11.01 ± 0.11 14.19 ± 0.175 32.86 ± 0.065 > 300 > 300 51.02 ± 0.04
SI NA > 16.78 NA 1.4 > 34.64 1.7 1.7 1.41 > 15.67 NA 0.57
Compound A11 B11 C11 D11 E11 F11 G11 H11 I11 J11 K11
K562 cell line > 100 39.24 ± 0.006 > 100 9.11 ± 0.23 13.65 ± 0.52 5.92 ± 0.002 > 100 8.46 ± 0.27 > 100 > 100 > 100
MCF-12A cell line 7.88 ± 0.01 > 300 > 300 10.15 ± 0.072 > 300 12.46 ± 0.053 97.93 ± 0.16 29.84 ± 0.17 > 300 > 300 28.31 ± 0.14
SI NA > 7.65 NA 1.1 > 21.98 2.1 NA 3.53 NA NA NA
  1. aIC50: concentration of the compound (μM) producing 50 % cell growth inhibition after 48 h of compound exposure, as determined by the WST-1 assay. Each experiment was run at least two times, and the results are presented as average values±standard deviation.