Table 2 Cytotoxicity activity of the synthesized individual compounds 1-11(A-K)
From: Schiff bases of indoline-2,3-dione (isatin) with potential antiproliferative activity
|  | Compound/IC50 (μM) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Compound | A1 | B1 | C1 | D1 | E1 | F1 | G1 | H1 | I1 | J1 | K1 |
K562 cell line | 20.04 ± 0.07 | 42.3 ± 0.03 | 13.47 ± 0.038 | 11.5 ± 0.01 | 6 ± 0.007 | 7.7 ± 0.02 | 5.66 ± 0.11 | 15.37 ± 0.02 | 11.59 ± 0.034 | > 100 | > 100 |
MCF-12A cell line | 69.23 ± 0.123 | 49.39 ± 0.16 | > 300 | > 300 | > 300 | 64.31 ± 0.026 | 16.61 ± 0.181 | 37.12 ± 0.27 | > 300 | > 300 | > 300 |
SI | 3.45 | 1.17 | > 22.27 | > 26.1 | > 50.00 | 8.4 | 2.9 | 2.42 | > 25.88 | NA | NA |
Compound | A2 | B2 | C2 | D2 | E2 | F2 | G2 | H2 | I2 | J2 | K2 |
K562 cell line | 49.13 ± 0.095 | 20.19 ± 0.09 | 29.82 ± 0.139 | 8.3 ± 0.003 | 7.76 ± 0.07 | 12.9 ± 0.002 | 5.72 ± 0.1 | 8.05 ± 0.035 | 8.67 ± 0.027 | 23.32 ± 0.32 | > 100 |
MCF-12A cell line | 32.55 ± 0.073 | > 300 | > 300 | 14.78 ± 0.4 | > 300 | 31.39 ± 0.096 | 16.62 ± 0.182 | 11.67 ± 0.003 | > 300 | 17.91 ± 0.62 | > 300 |
SI | 0.66 | > 14.86 | > 10.06 | 1.8 | > 38.66 | 2.4 | 2.9 | 1.45 | > 34.60 | 0.77 | NA |
Compound | A3 | B3 | C3 | D3 | E3 | F3 | G3 | H3 | I3 | J3 | K3 |
K562 cell line | 32.37 ± 0.074 | 19.35 ± 0.019 | 14.86 ± 0.055 | 13.86 ± 0.01 | 7.13 ± 0.009 | 14 ± 0.08 | 9.88 ± 0.41 | > 100 | 15.81 ± 0.11 | 19.75 ± 0.08 | > 100 |
MCF-12A cell line | 27.06 ± 0.272 | > 300 | > 300 | 258.09 ± 0.03 | > 300 | 8.71 ± 0.17 | 13.22 ± 0.034 | 97.36 ± 0.062 | 27.87 ± 0.07 | > 300 | 83.8± 0.039 |
SI | 0.84 | > 15.50 | > 20.19 | 18.6 | > 42.08 | 0.62 | 1.3 | NA | 1.76 | > 15.19 | NA |
Compound | A4 | B4 | C4 | D4 | E4 | F4 | G4 | H4 | I4 | J4 | K4 |
K562 cell line | 38.88 ± 0.138 | 51.51 ± 0.09 | 30.96 ± 0.2 | 9.98 ± 0.009 | 7.5 ± 0.03 | 16.28 ± 0.015 | 8.43 ± 0.163 | 24.21 ± 0.023 | 11.57 ± 0.051 | 14.52 ± 0.08 | > 100 |
MCF-12A cell line | 9.39 ± 0.152 | > 300 | > 300 | > 300 | > 300 | 50.85 ± 0.048 | 12.50 ± 0.216 | > 300 | > 300 | 12.74 ± 0.32 | 78.29 ± 0.31 |
SI | 0.24 | > 5.82 | > 9.69 | > 30 | > 40.00 | 3.1 | 1.5 | > 12.39 | > 25.93 | 0.88 | NA |
Compound | A5 | B5 | C5 | D5 | E5 | F5 | G5 | H5 | I5 | J5 | K5 |
K562 cell line | > 100 | 7.3 ± 0.01 | 23.9 ± 0.26 | 8.67 ± 0.007 | 10.3 ± 0.001 | 10.4 ± 0.044 | 6.5 ± 0.001 | 5.88 ± 0.004 | 24.11 ± 0.255 | 6.99 ± 0.005 | > 100 |
MCF-12A cell line | 39.74 ± 0.073 | > 300 | > 300 | 80.05 ± 0.40 | > 300 | 39.09 ± 0.24 | 9.1 ± 0.003 | 7.35 ± 0.094 | > 300 | 5.6 ± 0.003 | > 300 |
SI | NA | > 41.10 | > 12.55 | 9.2 | > 29.13 | 3.8 | 1.4 | 1.25 | > 12.44 | 0.80 | NA |
Compound | A6 | B6 | C6 | D6 | E6 | F6 | G6 | H6 | I6 | J6 | K6 |
K562 cell line | > 100 | > 100 | > 100 | 8.87 ± 0.017 | 9.58 ± 0.03 | 13.5 ± 0.03 | 6.62 ± 0.22 | 6.49 ± 0.017 | 8.55 ± 0.06 | 7.19 ± 0.051 | > 100 |
MCF-12A cell line | 13.91 ± 0.003 | > 300 | > 300 | 134.18 ± 0.142 | > 300 | 9.6 ± 0.15 | 13.05 ± 0.20 | 34.77 ± 0.062 | 26.15 ± 0.14 | 10.16 ± 1.2 | > 300 |
SI | NA | NA | NA | 15.1 | > 31.32 | 0.71 | 2 | 5.36 | 3.06 | 1.41 | NA |
Compound | A7 | B7 | C7 | D7 | E7 | F7 | G7 | H7 | I7 | J7 | K7 |
K562 cell line | > 100 | 47.25 ± 0.027 | > 100 | 9.1 ± 0.026 | 8.9 ± 0.02 | 12.86 ± 0.01 | 7.68 ± 0.34 | 23.67 ± 0.39 | > 100 | 18.02 ± 0.13 | > 100 |
MCF-12A cell line | 11.03 ± 0.151 | > 300 | > 300 | > 300 | > 300 | 11.39 ± 0.25 | 12.98 ± 0.439 | 73.18 ± 0.072 | > 300 | > 300 | 61.34 ± 0.01 |
SI | NA | > 6.35 | NA | > 33 | > 33.71 | 0.89 | 1.7 | 3.09 | NA | > 16.65 | NA |
Compound | A8 | B8 | C8 | D8 | E8 | F8 | G8 | H8 | I8 | J8 | K8 |
K562 cell line | > 100 | 27.23 ± 0.092 | > 100 | 6.9 ± 0.001 | 10.34 ± 0.015 | 6.99 ± 0.007 | 15.12 ± 0.48 | 40.65 ± 0.24 | > 100 | 45.51 ± 0.46 | > 100 |
MCF-12A cell line | 10.54 ± 0.157 | > 300 | > 300 | > 300 | > 300 | 21.29 ± 0.104 | > 300 | > 300 | > 300 | > 300 | > 300 |
SI | NA | > 11.02 | NA | > 43.5 | > 29.01 | 3 | > 19.8 | > 7.38 | NA | > 6.59 | NA |
Compound | A9 | B9 | C9 | D9 | E9 | F9 | G9 | H9 | I9 | J9 | K9 |
K562 cell line | 90.67 ± 0.19 | 17.42 ± 0.05 | > 100 | 6.2 ± 0.01 | 9.77 ± 0.01 | 6.77 ± 0.01 | > 100 | 26.72 ± 0.38 | 31.19 ± 0.004 | 52.05 ± 0.15 | > 100 |
MCF-12A cell line | 14.48 ± 0.647 | > 300 | > 300 | > 300 | > 300 | 13.14 ± 0.114 | > 300 | > 300 | > 300 | > 300 | 5.88 ± 0.11 |
SI | 0.16 | > 17.22 | NA | > 48.4 | > 30.71 | 1.9 | NA | > 11.23 | > 9.62 | > 5.76 | NA |
Compound | A10 | B10 | C10 | D10 | E10 | F10 | G10 | H10 | I10 | J10 | K10 |
K562 cell line | > 100 | 17.88 ± 0.062 | > 100 | 8.9 ± 0.43 | 8.66 ± 0.06 | 6.3 ± 0.005 | 8.43 ± 0.18 | 23.34 ± 0.19 | 19.14 ± 0.07 | > 100 | 88.85 ± 0.26 |
MCF-12A cell line | 8.33 ± 0.044 | > 300 | > 300 | 12.57 ± 0.089 | > 300 | 11.01 ± 0.11 | 14.19 ± 0.175 | 32.86 ± 0.065 | > 300 | > 300 | 51.02 ± 0.04 |
SI | NA | > 16.78 | NA | 1.4 | > 34.64 | 1.7 | 1.7 | 1.41 | > 15.67 | NA | 0.57 |
Compound | A11 | B11 | C11 | D11 | E11 | F11 | G11 | H11 | I11 | J11 | K11 |
K562 cell line | > 100 | 39.24 ± 0.006 | > 100 | 9.11 ± 0.23 | 13.65 ± 0.52 | 5.92 ± 0.002 | > 100 | 8.46 ± 0.27 | > 100 | > 100 | > 100 |
MCF-12A cell line | 7.88 ± 0.01 | > 300 | > 300 | 10.15 ± 0.072 | > 300 | 12.46 ± 0.053 | 97.93 ± 0.16 | 29.84 ± 0.17 | > 300 | > 300 | 28.31 ± 0.14 |
SI | NA | > 7.65 | NA | 1.1 | > 21.98 | 2.1 | NA | 3.53 | NA | NA | NA |