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Table 2 Validation report of the proposed second derivative spectrofluorimetric method for determination of MLT

From: New spectrofluorimetric methods for determination of melatonin in the presence of N-{2-[1-({3-[2-(acetylamino)ethyl]-5-methoxy-1H-indol-2-yl}methyl)-5-methoxy-1H-indol-3-yl]- ethyl}acetamide: a contaminant in commercial melatonin preparations

Parameters

MLT

Linear range (ng ml−1)

20-220

Intercept ± SD

0.28 ± 0.906

Slope ± SD

1.001 ± 0.008

Correlation Coefficient

0.9995

LOD (ng ml-1)

2.988

LOQ (ng ml-1)

9.055

Accuracya

100.70 ± 1.772

Repeatabilitya

99.81.32 ± 1.799

Intermediate precisiona

100.83 ± 2.445

  1. a corresponding values are average of three determinations ± SD.