Figure 2
From: Cleavage of pyrene-stabilized RNA bulge loops by trans-(±)-cyclohexane-1,2-diamine
Cleavage of RNA 1 in buffers containing 20 mM NaCl, 1 mM Tris-EDTA (pH 8.0), and different organic catalysts (0.5 M, pH 8.0). Lane 1, no catalyst. Lane 2, trans-(±)-cyclohexane-1,2-diamine. Lane 3, glycine, Lane 4, propane-1,3-diamine. Lane 5, ethylenediamine. Lane 6, imidazole. Overall cleavage: Lane 1, 0%. Lane 2, 31%. Lane 3, 0%. Lane 4, 5%. Lane 5, 24%. Lane 6, < 1%.