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Table 2 The Ca2+ Binding constants for the 1st and 2nd EF-hands

From: Characterization of the 1st and 2nd EF-hands of NADPH oxidase 5 by fluorescence, isothermal titration calorimetry, and circular dichroism

Protein

Ka M-1 ( ΔH kJ/mol)

NCaBDa

1.7 -6.8 × 104

NCaBDb

3.05 ± 1.02 × 105 (-4.96 ± 0.25), 8.60 ± 1.65 × 106 (-24.88 ± 0.43)

-1EF + 2EF

6.51 ± 0.80 × 105 (-14.3 ± 0.3)

+1EF-2EF c

~ 5 × 102 (-4.0 ± 0.2)

-1EF-2EF

N/Dd

  1. aThe data is from ref. [6].
  2. bThe ITC data was fitted with a model with two independent binding sites.
  3. cThe binding constant was estimated from fluorescence and ITC, assuming two Ca2+ binding sites.
  4. dNot detected.
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BMC Chemistry

ISSN: 2661-801X

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