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Table 1 Stern-Volmer Quenching Constants Obtained from Acrylamide Titration

From: Characterization of the 1st and 2nd EF-hands of NADPH oxidase 5 by fluorescence, isothermal titration calorimetry, and circular dichroism

Ksv (M-1)

Protein

apo

Mg2+

Ca2+

CaBD

5.28 ± 0.22

5.04 ± 0.35

9.49 ± 0.52

NCaBDa

6.95 ± 0.22

6.78 ± 0.35

12.66 ± 0.52

-1EF+2EFa

6.61 ± 0.17

6.68 ± 0.33

9.28 ± 0.08

+1EF-2EFa

6.70 ± 0.21

6.71 ± 0.15

9.51 ± 0.16

-1EF-2EFa

7.40 ± 0.42

7.35 ± 0.35

7.45 ± 0.41

  1. aProteins were excited at 280 nm and their intensities at the maximum wavelengths were recorded. The concentrations of EDTA, Mg2+, and Ca2+ were 0.5, 10, and 1 mM (4 and 10 mM for +1EF-2EF and -1EF-2EF, respectively).
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BMC Chemistry

ISSN: 2661-801X

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