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Table 1 Stern-Volmer Quenching Constants Obtained from Acrylamide Titration

From: Characterization of the 1st and 2nd EF-hands of NADPH oxidase 5 by fluorescence, isothermal titration calorimetry, and circular dichroism

Ksv (M-1)
Protein apo Mg2+ Ca2+
CaBD 5.28 ± 0.22 5.04 ± 0.35 9.49 ± 0.52
NCaBDa 6.95 ± 0.22 6.78 ± 0.35 12.66 ± 0.52
-1EF+2EFa 6.61 ± 0.17 6.68 ± 0.33 9.28 ± 0.08
+1EF-2EFa 6.70 ± 0.21 6.71 ± 0.15 9.51 ± 0.16
-1EF-2EFa 7.40 ± 0.42 7.35 ± 0.35 7.45 ± 0.41
  1. aProteins were excited at 280 nm and their intensities at the maximum wavelengths were recorded. The concentrations of EDTA, Mg2+, and Ca2+ were 0.5, 10, and 1 mM (4 and 10 mM for +1EF-2EF and -1EF-2EF, respectively).