Time-lapse SECM images presenting the changing morphology of a live T24 cell under room temperature taken with a 5 μm diameter Pt UME. The scanning area of each image was 80 × 60 μm (a-d)/80 × 80 μm (e-p). The acquisition time of each image was about 3 min (256 × 256 pixels). Time-lapse cross section lines extracted from Figure 3a-p present the changing topography of the T24 cell under the room temperature. Each cross section line goes through the center of the cell nucleus. Background tilt was adjusted. Red arrows: shrinking cellular membrane. Green triangles: non-nucleus part of the great nuclear area. Yellow triangles: nucleus part of the great nuclear area.