Figure 3

Effects of the Cu(II) complexes on the transcriptional activity of AhR and GR. AZ-GR (Panel A) and/or AZ-AHR (Panel A) cells were seeded on 96-well plates and stabilized for 16 h. Thereafter, the cells were treated for 24 h with the tested complexes (10 nM – 5 μM), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 10 pM – 10 μM), dexamethasone (DEX; 10 pM – 100 nM) and/or vehicle (DMSO; 0.1% V/V). After the treatments, the cells were lysed and the luciferase activity was measured. The data are mean from triplicate measurements and are expressed as a fold induction over the DMSO-treated cells. Experiments were performed in two different passages of the AZ-AHR and AZ-GR cells. The differences between the individual measurements were lower that 5%.