On-line solid-phase enrichment coupled to packed reactor flow injection analysis in a green analytical procedure to determine low levels of folic acid using fluorescence detection

Table 1 Linearity parameters and intra- and inter-day validation of the method to determine FA in freshly prepared tablets

Linearity parameters^a		Concentration (ng/mL)	Intra-assay	Intra-assay
Calibration range (ng/mL)	1.25-50	Concentration (ng/mL)	Mean recovery^b (% ± RSD)	Mean recovery^b (% ± RSD)
Detection limit (ng/mL)	0.49	5	98.88 ± 0.62	98.45 ± 0.81
Slope (b)	1.4317	15	99.15 ± 0.34	99.01 ± 0.44
Standard error of the slope	0.0094	30	99.27 ± 0.44	99.13 ± 0.53
Intercept (a)	0.3517	45	99.14 ± 0.51	98.92 ± 0.59
Standard error of the intercept	0.2319		Average
Correlation coefficient (r²)	0.9997		99.11 ± 0.48	98.88 ± 0.59

^a Y = a + bC, where C is the concentration of FA in ng/mL and Y is the peak area.
^b Values are mean of five determinations.

ISSN: 2661-801X