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Figure 6 | Chemistry Central Journal

Figure 6

From: On-line solid-phase enrichment coupled to packed reactor flow injection analysis in a green analytical procedure to determine low levels of folic acid using fluorescence detection

Figure 6

Diagram chart and calibration curve (inset) for on-line pre-column derivatization combined by SPEn to determine FA over the concentration range of 1.25-50 ng/mL. Experimental conditions: phosphate buffer (0.04M, pH 3.4) at a flow-rate of 0.25 mL/min and CTH reactor temperature of 40°C in derivatization and enrichment step; mobile phase consisting of ethanol and phosphate buffer (0.04M, pH 3.4) in the ratio of 5:95 (v/v) at a flow-rate of 1 mL/min for back-flush elution step.

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