Figure 5

Effect of compounds 3, 4, 7, 8 and 9 on COX-1 expression in COS-7 cells. COS-7 transfected cells were incubated for 30 minutes with 25 or 50 μM of compounds 3, 4, 7, 8 and 9 prior to the addition of 25 μM of arachidonic acid (AA) for an additional 30 minutes, after which cells were lysed. Proteins of non-transfected and transfected cells were loaded on a 10% SDS-polyacrylamide gel and blotted for COX-1 and β-actin expression. Densitometry analysis was performed and ratio of COX-1/β-actin was calculated. Results are representative of 2 experiments with similar results.