Skip to main content

Table 3 Comparison of three columns under optimized conditions

From: Development and validation of a chromatographic method for determining Clematichinenoside AR and related impurities

Columns Compounds Rs As α k’ NTP
Thermo ODS Hypersil Imp-1 - 1.681 - 0.279 8610
(4.6mm ×150mm, 5 μm) Imp −2 3.62 1.281 1.234 0.579 3280
AR 9.49 0.879 2.583 2.178 3162
Imp-3 1.60 1.036 1.119 2.559 3255
Imp-4 1.63 0.934 1.116 2.979 3615
Imp-5 40.03 2.778 3.215 10.974 127412
Diamonsil C18(4.6mm ×150mm, 5 μm) Imp-1 - 0.707 - 0.237 5722
Imp-2 3.37 1.135 1.283 0.586 2009
AR 9.41 0.878 3.051 2.786 2167
Imp-3 1.52 0.977 1.135 3.297 2448
Imp-4 1.90 0.951 1.157 3.977 2948
Imp-5 27.88 0.691 2.341 10.740 159346
Agilent TC-C18(4.6mm×150mm,5 μm) Imp-1 - 1.124 - 0.693 4009
Imp-2 6.79 1.047 1.872 1.630 3889
AR 18.33 0.838 2.856 5.547 10299
Imp-3 1.99 0.964 1.080 6.032 14992
Imp-4 2.06 0.974 1.070 6.492 19418
  Imp-5 26.91 1.012 1.686 11.335 248207
  1. Rs, resolution; As, tailing factor; α, selectivity; k’, capacity factor; NTP, number of theoretical plates.