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Table 1 The IC 50 values of five RC extracts and the positive controls (μg/mL)

From: Antioxidant activity and mechanism of Rhizoma Cimicifugae

Assays PERC EARC AERC 95ERC WRC GSH
Anti-lipid peroxidation 13.99 ± 2.11e 4.55 ± 0.44 b 14.92 ± 4.31 d 10.62 ± 0.97 c 19.18 ± 2.04 f 0.028 ± 0.00 a *
DNA protective effect - 1905.51 ± 177.54 c 14397.18 ± 1075.01 d 1090.11 ± 21.25 b - 114.42 ± 2.66 a **
·OH 561.19 ± 21.16 f 111.55 ± 1.32 c 132.32 ± 0.15 d 84.30 ± 1.02 b 233.45 ± 15.70 e 37.67 ± 0.67 a **
·O2- 322.81 ± 14.90 b 306.68 ± 0.87 b 284.36 ± 11.43 b 301.08 ± 8.34 b 650.65 ± 59.55 c 81.65 ± 5.21 a
Chelating 191.86 ± 1.84 b 319.20 ± 6.36 c 872.71 ± 30.63 e 553.34 ± 12.59 d 2289.11 ± 100.00 f 107.86 ± 0.31 a ***
DPPH· 813.00 ± 225.17 d 227.09 ± 10.14 b 260.34 ± 11.37 c 254.73 ± 4.33 c 229.17 ± 5.81 b 5.13 ± 1.19 a **
ABTS·+ 395.65 ± 77.34 e 72.10 ± 1.32 b 96.39 ± 1.12 d 94.38 ± 2.45 d 83.58 ± 0.81 c 4.76 ± 0.28 a
Fe3+ reducing 533.12 ± 54.25 e 159.35 ± 1.37 b 317.06 ± 3.47 c 156.19 ± 2.12 b 488.75 ± 18.6 0 d 51.89 ± 1.29 a
  1. IC50 value is defined as the concentration of 50% effect percentage and calculated by linear regression analysis and expressed as mean ± SD (n = 3). The linear regression was analyzed by Origin 6.0 professional software. Means values with different superscripts in the same row are significantly different (p < 0.05), while with same superscripts are not significantly different (p < 0.05). *The positive control was BHA, instead of GSH. ** The positive control was Trolox, instead of GSH. *** The positive control was Sodium citrate. Results were analyzed by independent samples. PERC, petroleum ether extract of rhizoma Cimicifugae; EARC, ethyl acetate extract of rhizoma Cimicifugae; AERC, absolute ethanol extract of rhizoma Cimicifugae; 95ERC, 95% ethanol extract of rhizoma Cimicifugae; WRC, water extract of rhizoma Cimicifugae. --: Cannot be detected.