Figure 9
From: Alpha chymotrypsin coated clusters of Fe3O4nanoparticles for biocatalysis in low water media
Far UV circular dichroism spectra of EPRP and ECCN 3 suspended in n -propanol. The spectra was recorded using a spinning cell holder as an accessory and of native alpha chymotrypsin in 50 mM sodium phosphate buffer, pH 7.8. Native alpha chymotrypsin (1 mg mL-1) solution in the aqueous buffer was used for recording the spectrum. In case of the EPRP and ECCN 3, after the CD spectra had been recorded, the suspensions were collected from the cell and redissolved in the same buffer by gentle vortexing. The superparamagnetic nanoparticles were separated by a magnetic separator and the clear supernatant containing the dissolved protein was used to find the enzyme concentration by measuring its absorbance at 280 nm. These concentrations were used to calculate mean residual ellipticity (MRE) values.