Figure 8

% alpha chymotrypsin precipitated as free EPRP and onto the nanoclusters. The ECCNs were separated using a magnetic separator wherein the free EPRPs remained behind but the protein coated nanoclusters were attracted to the magnet. Thus the free EPRPs were collected and centrifuged at 5000g for 5 min. The supernatant was removed and EPRPs were redissolved back in 50 mM sodium phosphate buffer, pH 7.8 and the protein was measured by absorbance at 280 nm. The separated ECCN was also similarly suspended back in the aqueous buffer, gently vortexed and the superparamagnetic nanoparticles were separated by a magnetic separator. The clear supernatant was then measured at 280 nm for the protein which was coated onto the nanoclusters. Each measurement was done in duplicate and the difference between each reading was within 3%.