Skip to main content

Table 1 Optimization of variables affecting the reaction of LND with FLC

From: A highly sensitive fluorimetric method for determination of lenalidomide in its bulk form and capsules via derivatization with fluorescamine

Variable

Studied range

Optimum condition

FLC conc. (%, w/v)

0.00025 – 0.0045

0.0025

pH

6.5–9.5

7 ± 0.2

Reaction time (min)

2–30

10

Solvent

Different a

Water

Stability of LND-FLC (min)

10–60

10–60

Excitation wavelength, λex (nm)

220–460

381

Emission wavelength, λem (nm)

420–620

494

  1. a Solvents tested: Water, methanol, ethanol, and acetonitrile.
  2. b The stability of the LND-FLC was studied after dilution of the reaction solution.