Table 1 Validation results for vitamin D assay
Analytes in(nmol/L) | Conctrn.QC's | Linear range | LOD | r2 | Intra-day | Inter-day | ||
|---|---|---|---|---|---|---|---|---|
Precision % CV | Accuracy% | Precision % CV | Accuracy% | |||||
Vitamin D3 | 20 | 0.5-100 | 0.05 | 0.9997 | 4.13 | 87.1 | 5.32 | 89.8 |
40 | 2.86 | 99.4 | 2.33 | 89.4 | ||||
80 | 1.31 | 98.9 | 2.89 | 90.2 | ||||
Vitamin D2 | 20 | 0.5-100 | 0.05 | 0.9996 | 6.3 | 99.2 | 11.3 | 93.2 |
40 | 11.3 | 97.5 | 13.3 | 94.8 | ||||
80 | 9.4 | 89.4 | 9.6 | 89.3 | ||||
25OHD3 | 20 | 0.5-100 | 0.025 | 0.9999 | 6.1 | 99.9 | 7.4 | 98.1 |
40 | 2.7 | 97.3 | 3.7 | 99.8 | ||||
80 | 3.5 | 99.1 | 2.9 | 99.2 | ||||
25OHD2 | 20 | 0.5-100 | 0.025 | 0.9998 | 2.8 | 99.8 | 3.4 | 101.2 |
40 | 2.7 | 99.9 | 3.9 | 98.9 | ||||
80 | 3.3 | 88.7 | 3.8 | 89.3 | ||||
1α25(OH)2 D3 | 0.2 | 0.015-1 | 0.010 | 0.9998 | 4.4 | 89.2 | 5.6 | 88.5 |
0.4 | 4.2 | 86.4 | 4.4 | 87.3 | ||||
0.8 | 3.6 | 99.7 | 2.6 | 88.9 | ||||
1α25(OH)2 D2 | 0.2 | 0.015-1 | 0.010 | 0.9998 | 3.2 | 98.9 | 5.2 | 89.8 |
0.4 | 4.4 | 99.4 | 6.3 | 98.9 | ||||
0.8 | 3.9 | 99.9 | 4.3 | 87.8 | ||||
3-epi-25OHD3 | 20 | 0.1-100 | 0.05 | 0.9997 | 3.5 | 89.2 | 4.3 | 98.5 |
40 | 3.1 | 87.3 | 10.9 | 87.6 | ||||
80 | 3.3 | 89.1 | 8.9 | 99.9 | ||||
3-epi-25OHD2 | 20 | 0.1-100 | 0.05 | 0.9998 | 7.1 | 85.8 | 3.5 | 88.5 |
40 | 4.9 | 84.6 | 2.7 | 87.1 | ||||
80 | 3.2 | 89.9 | 2.2 | 88.3 | ||||
7αC4 | 20 | 0.5-100 | 0.1 | 0.9999 | 3.2 | 98.3 | 5.4 | 86.2 |
40 | 2.3 | 88.6 | 3.1 | 85.3 | ||||
80 | 4.1 | 79.9 | 3.1 | 88.1 | ||||