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Table 1 Validation results for vitamin D assay

From: Method for simultaneous analysis of eight analogues of vitamin D using liquid chromatography tandem mass spectrometry

Analytes in(nmol/L) Conctrn.QC's Linear range LOD r2 Intra-day Inter-day
   Precision % CV Accuracy% Precision % CV Accuracy%
Vitamin D3 20 0.5-100 0.05 0.9997 4.13 87.1 5.32 89.8
40 2.86 99.4 2.33 89.4
80 1.31 98.9 2.89 90.2
Vitamin D2 20 0.5-100 0.05 0.9996 6.3 99.2 11.3 93.2
40 11.3 97.5 13.3 94.8
80 9.4 89.4 9.6 89.3
25OHD3 20 0.5-100 0.025 0.9999 6.1 99.9 7.4 98.1
40 2.7 97.3 3.7 99.8
80 3.5 99.1 2.9 99.2
25OHD2 20 0.5-100 0.025 0.9998 2.8 99.8 3.4 101.2
40 2.7 99.9 3.9 98.9
80 3.3 88.7 3.8 89.3
1α25(OH)2 D3 0.2 0.015-1 0.010 0.9998 4.4 89.2 5.6 88.5
0.4 4.2 86.4 4.4 87.3
0.8 3.6 99.7 2.6 88.9
1α25(OH)2 D2 0.2 0.015-1 0.010 0.9998 3.2 98.9 5.2 89.8
0.4 4.4 99.4 6.3 98.9
0.8 3.9 99.9 4.3 87.8
3-epi-25OHD3 20 0.1-100 0.05 0.9997 3.5 89.2 4.3 98.5
40 3.1 87.3 10.9 87.6
80 3.3 89.1 8.9 99.9
3-epi-25OHD2 20 0.1-100 0.05 0.9998 7.1 85.8 3.5 88.5
40 4.9 84.6 2.7 87.1
80 3.2 89.9 2.2 88.3
7αC4 20 0.5-100 0.1 0.9999 3.2 98.3 5.4 86.2
40 2.3 88.6 3.1 85.3
80 4.1 79.9 3.1 88.1