Table 1 Purification summary for laccase produced by P . ostreatus IBL-02 during decolorization of DBR K- 4BL
Purification steps | Total volume (mL) | Total enzyme activity (U) | Total protein content (mg) | Specific activity (U/mg) | % Yield | Purification fold |
|---|---|---|---|---|---|---|
Crude Enzyme | 500 | 62500 | 2360 | 134.95 | 1 | 100 |
(NH4)2SO4 | 30 | 48060 | 138 | 348.26 | 2.5 | 76 |
Precipitation | ||||||
DEAE-Sepharose | 12 | 9440 | 20.34 | 464.11 | 3.4 | 15 |
Sephadex-G-100 | 09 | 7020 | 12.24 | 573.52 | 4.2 | 11 |