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Table 1 Purification summary for laccase produced by P . ostreatus IBL-02 during decolorization of DBR K- 4BL

From: Improvement of Catalytic Efficiency, Thermo-stability and Dye Decolorization Capability of Pleurotus ostreatusIBL-02 laccase by Hydrophobic Sol Gel Entrapment

Purification steps Total volume (mL) Total enzyme activity (U) Total protein content (mg) Specific activity (U/mg) % Yield Purification fold
Crude Enzyme 500 62500 2360 134.95 1 100
(NH4)2SO4 30 48060 138 348.26 2.5 76
Precipitation
DEAE-Sepharose 12 9440 20.34 464.11 3.4 15
Sephadex-G-100 09 7020 12.24 573.52 4.2 11