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Table 2 SIM, regression data, LOD, LOQ and recovery of 11 investigated compounds analyzed by GC-MS

From: Comparison of sterols and fatty acids in two species of Ganoderma

Analytes

SIM

Linear regression data

R2

LOD

(ng)

LOQ

(ng)

Recoverya

(%, n = 3)

  

Regressive Equation Linear range (ng)

    

Lauric acid

257

y = 55788x - 7172

0.2-4.7

0.9991

0.070

0.132

99.5

Myristic acid

285

y = 92491x - 9626

0.2-4.7

0.9994

0.039

0.073

99.9

Pentadecanoic acid

299

y = 100984x - 19569

0.3-8.6

0.9998

0.065

0.123

98.5

Palmitoieic acid

311

y = 107565x - 28765

0.4-11.8

0.9994

0.084

0.231

99.1

Palmitic acid

313

y = 13939x - 713

0.5-15.1

0.9990

0.064

0.118

101.4

Linoleic acid

337

y = 16543x - 9768

1.3-6.3

0.9982

0.220

0.413

101.3

Oleic acid

339

y = 36027x - 9874

0.4-7.0

0.9992

0.119

0.155

101.1

Stearic acid

341

y = 68023x - 67

0.3-37.7

0.9995

0.141

0.268

96.9

Docosanoic acid

397

y = 103013x - 88418

1.5-4.9

0.9990

0.485

0.971

100.2

Lignoceric acid

425

y = 103037x - 35337

0.6-9.3

0.9984

0.223

0.527

97.3

Ergosterol

363

y = 40732x - 6971

0.5-3.8

0.9989

0.251

0.471

101.9

  1. a Recovery (%) = (amount found-original amount)/amount spiked.