Spectrophotometric determination of certain CNS stimulants in dosage forms and spiked human urine via derivatization with 2,4-Dinitrofluorobenzene

Table 4 Application of the proposed and comparison methods to the determination of the studied drugs in dosage form

Parameter	The proposed method		Comparison method⁽³⁵⁾
	Conc. taken (μg/mL)	% Found^a	% Found
Contaflu tablets ^c (24 mg of PPA, 3 mg of chlorpheniramine maleate and 200 mg of propyphenazone/tablet)	8.0	99.92	99.77⁽³⁵⁾
	12.0	99.47	99.36
	14.0	99.87	100.60
$\bar{x}$ ± S.D.	99.75^d ± 0.25		99.91 ± 0.63
T	0.588 (2.776)*
F	6.350 (19.00)*
Ephedrine mpoule ^d (30 mg of EPH/Ampoule)	7.0	100.57	100.73⁽³⁶⁾
	8.0	100.87	101.44
	10.0	101.91	102.36
$\bar{x}$ ± S.D.	101.12^e ± 0.70		100.51 ± 0.82
T	0.647 (2.776)*
F	1.372 (19.00)*
Allercet ^® capsule ^b (30 mg PSE and 10 mg cetirizine HCl/capsule)	6.0	100.18	100.98⁽³⁷⁾
	10.0	101.40	99.56
	12.0	102.04	102.15
$\bar{x}$ ± S.D.	101.21^f ± 0.94		100.90 ± 1.30
t	0.334 (2.776)*
F	1.913 (19.00)*

^a Each result is the average of three separate determinations.
*Values between parentheses are the tabulated t and F values, at p 0.05⁽³⁸⁾.
^a Contaflu tablets, batch # 061342, each tablet labeled to contain 24 mg of phenylpropanolamine HCl, 3 mg of chlorpheniramine maleate and 200 mg of propyphenazone, product of Egyptian Int..Pharmaceutical Industries CO. E.P.I.CO,10^th of Ramadan City, Egypt.
^b Ephedrine ampoule:-batch # 11 each ampoule (1 mL) labeled to contain 30 mg of ephedrine HCl, A product of Chemical industries Development(CID)-Giza-A.R.E.
^c Allercet capsule, batch # 820304, each capsule labeled to contain 30 mg pseudoephedrine HCl and 10 mg cetirizine HCl, product of Global Napi Pharmaceuticals, 6^th of October City-Giza-Egypt.
^d PPA content found in Contaflu tablet was found to be 23.94 mg/tablet.
^e EPH content found in Ephedrine ampoule was found to be 30.34 mg/ampoule.
^f PSE content found in Allercet^® capsule was found to be 30.36 mg/capsule.

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