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Table 1 Performance data of the proposed method.

From: Development and validation of stability indicating method for determination of sertraline following ICH guidlines and its determination in pharmaceuticals and biological fluids

Parameter

Spectrofluorimetric Method

Spectrophotometric Method

Ref. method [23]

-concentration range (μg/mL ).

0.25-5

2-24

 

-LOD (μg/mL ).

0.069

0.184

 

-LOQ (μg/mL).

0.209

0.558

 

-Correlation coefficient ( r).

0.9999

0.9999

 

-Slope

31.8

0.035

 

-Intercept

19.038

0.039

 

-Sy/x

0.928

2.72 × 10-3

 

-Sa

0.664

1.96 × 10-3

 

-Sb

0.221

1.387 × 10-4

 

-% Error

0.29

0.21

 

-%RSD

0.65

0.51

 

-No.of Experiments.

5

6

3

-Mean found (%)

100.12

100.74

100

± SD.

0.65

0.51

0.31

-Student's t-value.

0.275 (2.45)

2.216 (2.37)

 

-Variance ratio F-test.

4.352 (19.25)

2.762 (19.30)

 

-Applications.

tablet preparations and

spiked human plasma

 
  1. N.B.
  2. -Sy/x =standard deviation of the residuals.
  3. -Sa = standard deviation of the intercept of regression line.
  4. -Sb = standard deviation of the slope of regression line
  5. -% Error = RSD%/√ n.
  6. - Figures between parentheses are the tabulated t and F values respectively, at p = 0.05.[25]