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Table 1 Example of Working Standard Dilution Scheme(s)

From: An industry consensus study on an HPLC fluorescence method for the determination of (±)-catechin and (±)-epicatechin in cocoa and chocolate products

Working Standard Use one of the stock standard solution and dilute to prepare working standards in 10 mL volumetric flasks
1000 µg/mL (Standard A); or 100 µg/mL (Standard B);
[mL]
Stock Standard A
Approximate Working Standard Concentration
[µg/mL]
Stock Standard B
Approximate Working Standard Concentration
[µg/mL]
Blank 0 0 0
1 0.250 25 2.5
2 1.000 50 5.0
3 2.000 200 20.0
4 3.000 300 30.0
5 4.000 400 40.0
6 5.000 500 50.0
7 Use "as is" 1000 100.0