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Table 1 Example of Working Standard Dilution Scheme(s)

From: An industry consensus study on an HPLC fluorescence method for the determination of (±)-catechin and (±)-epicatechin in cocoa and chocolate products

Working Standard

Use one of the stock standard solution and dilute to prepare working standards in 10 mL volumetric flasks

1000 µg/mL (Standard A); or 100 µg/mL (Standard B);

[mL]

Stock Standard A

Approximate Working Standard Concentration

[µg/mL]

Stock Standard B

Approximate Working Standard Concentration

[µg/mL]

Blank

0

0

0

1

0.250

25

2.5

2

1.000

50

5.0

3

2.000

200

20.0

4

3.000

300

30.0

5

4.000

400

40.0

6

5.000

500

50.0

7

Use "as is"

1000

100.0