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Table 1 Comparison of some conditions and features of the SIC method with previous HPLC methods

From: Sequential injection chromatography with a miniaturized multi-channel fiber optic detector for separation and quantification of propranolol and hydrochlorothiazide

Condition

SIC

HPLC [20]

HPLC [22]

Separation column

C18 (4.6 × 50 mm)

CPSa (4.6 × 25 mm)

C18 (4.6 × 150 mm)

Mobile phase composition

75 mmol/L PHS:ACNb (70:30, v/v) at pH 3.0

50 mmol/L PHS:ACN (85:15, v/v) at pH 3.0

75 mmol/L PHS:ACN (70:30, v/v) at pH 3.0

Consumed mobile phase volume (mL)

4

< 10

-

Sample volume (μL)

40

20

20

Waste production volume (mL)

4

10

-

Flow rate (mL/min)

1.8

2.0

1.2

Analysis time (s)

165

< 1200

-

Resolution

3.6

12.1

8.9

 

HTZ

PRP

HTZ

PRP

HTZ

PRP

UV detection (nm)

270

290

290

290

274

290

Retention time (s)

0.7

1.0

4.5

14.2

2.2

5.7

Peak symmetry

1.10

1.50

-

-

0.98

0.57

Theoretical plates

4203

88671

8962

8962

172

385

Calibration equation

PHc = 0.0231Cd+0.0398

PH = 0.0028C+0.095

-

-

PH = 0.022C

+0.0144

PH = 0.0129C+0.0283

Correlation coefficient

0.9999

0.9989

1.0000

0.9993

0.9995

0.9990

Recovery (%)

97.0-100.5

94.0-104.9

98.0-100.4

97.8-100.3

-

-

Linear range (μg/mL)

5-50

25-200

10-92

16-160

1-70

2-40

LOD (μg/mL)

0.6

1.0

-

-

0.6

0.3

LOQ (μg/mL)

2.0

3.4

-

-

2.0

1.0

Repeatability (RSD %)

0.77

0.40

0.30

0.70

-

-

  1. a: Cyanopropylsilane; b: phosphate:acetonitrile; c: peak height; d: concentration (μg/mL)