Condition | SIC | HPLC [20] | HPLC [22] | |||
---|---|---|---|---|---|---|
Separation column | C18 (4.6 × 50 mm) | CPSa (4.6 × 25 mm) | C18 (4.6 × 150 mm) | |||
Mobile phase composition | 75 mmol/L PHS:ACNb (70:30, v/v) at pH 3.0 | 50 mmol/L PHS:ACN (85:15, v/v) at pH 3.0 | 75 mmol/L PHS:ACN (70:30, v/v) at pH 3.0 | |||
Consumed mobile phase volume (mL) | 4 | < 10 | - | |||
Sample volume (μL) | 40 | 20 | 20 | |||
Waste production volume (mL) | 4 | 10 | - | |||
Flow rate (mL/min) | 1.8 | 2.0 | 1.2 | |||
Analysis time (s) | 165 | < 1200 | - | |||
Resolution | 3.6 | 12.1 | 8.9 | |||
 | HTZ | PRP | HTZ | PRP | HTZ | PRP |
UV detection (nm) | 270 | 290 | 290 | 290 | 274 | 290 |
Retention time (s) | 0.7 | 1.0 | 4.5 | 14.2 | 2.2 | 5.7 |
Peak symmetry | 1.10 | 1.50 | - | - | 0.98 | 0.57 |
Theoretical plates | 4203 | 88671 | 8962 | 8962 | 172 | 385 |
Calibration equation | PHc = 0.0231Cd+0.0398 | PH = 0.0028C+0.095 | - | - | PH = 0.022C +0.0144 | PH = 0.0129C+0.0283 |
Correlation coefficient | 0.9999 | 0.9989 | 1.0000 | 0.9993 | 0.9995 | 0.9990 |
Recovery (%) | 97.0-100.5 | 94.0-104.9 | 98.0-100.4 | 97.8-100.3 | - | - |
Linear range (μg/mL) | 5-50 | 25-200 | 10-92 | 16-160 | 1-70 | 2-40 |
LOD (μg/mL) | 0.6 | 1.0 | - | - | 0.6 | 0.3 |
LOQ (μg/mL) | 2.0 | 3.4 | - | - | 2.0 | 1.0 |
Repeatability (RSD %) | 0.77 | 0.40 | 0.30 | 0.70 | - | - |