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Table 1 In vitro bioactivity of mono-PEGylated forms of TNF-αs

From: Development of a novel DDS for site-specific PEGylated proteins

  EC50 (ng/ml) (% of remaining activity)
wTNF 0.17
mTNF-K90R 0.03
ran-PEG-wTNF 2.85 (6.0%)
sp-PEG-mTNF-K90R 0.05 (60.0%)
  1. The specific activity of the mono-PEGylated forms of TNF-α was measured by a cytotoxic assay using LM cells in the presence of actinomycin D. EC50 is the concentration of various PEGylated TNF-αs capable of killing 50% of the cells.