Development of a novel DDS for site-specific PEGylated proteins

BMC Chemistry

Table 1 In vitro bioactivity of mono-PEGylated forms of TNF-αs

	EC50 (ng/ml) (% of remaining activity)
wTNF	0.17
mTNF-K90R	0.03
ran-PEG-wTNF	2.85 (6.0%)
sp-PEG-mTNF-K90R	0.05 (60.0%)

The specific activity of the mono-PEGylated forms of TNF-α was measured by a cytotoxic assay using LM cells in the presence of actinomycin D. EC₅₀ is the concentration of various PEGylated TNF-αs capable of killing 50% of the cells.

ISSN: 2661-801X