Parameter | K' | N | HETP | Rs | Peak area ratio |
---|---|---|---|---|---|
Buffer type and pH | |||||
Britton Robinson buffer pH | |||||
3 | 3.41 | 1036 | 0.0145 | 2.73 | 0.726 |
3.5 | 3.92 | 1034 | 0.0145 | 3.58 | 0.743 |
4 | 5.53 | 2706 | 0.0055 | 5.012 | 0.784 |
4.5 | 5.13 | 2698 | 0.0055 | 5.012 | 0.784 |
5 | 3.84 | 1507 | 0.0099 | 4.177 | 0.752 |
5.5 | 3.66 | 769 | 0.0195 | 3.166 | 0.688 |
6 | 3.76 | 258 | 0.0581 | 3.133 | 0.564 |
Phosphate buffer pH 4.2 | 4.26 | 1479 | 0.0101 | 2.72 | 0.366 |
Acetate buffer pH 4.2 | 4.06 | 1027 | 0.0146 | 1.271 | 0.521 |
Ratio of aqueous: organic phase(acetonitril: methanol; 1:4) | |||||
45:55 | 4.02 | 837 | 0.0179 | 1.088 | 0.29 |
40:60 | 4.25 | 1034 | 0.0145 | 4.15 | 0.45 |
35:65 | 5.53 | 2706 | 0.0055 | 5.012 | 0.784 |
30:70 | 5.52 | 2687 | 0.0056 | 5.009 | 0.783 |
Ratio of acetonitril: methanol | |||||
1:4 | 5.53 | 2706 | 0.0055 | 5.012 | 0.784 |
2:3 | 3.52 | 1210 | 0.0124 | 3.64 | 0.645 |
3:2 | 2.73 | 2205 | 0.0068 | 4.45 | 0.429 |
4:1 | 4.103 | 1393 | 0.0108 | 4.41 | 0.235 |
Acetonitril only | 5.08 | 1347 | 0.0111 | 4.745 | 0.198 |
Methanol only | 4.938 | 1817 | 0.0083 | 4.371 | 0.349 |
Ionic strength of BRb (Molar) | |||||
0.0075 | 4.256 | 911 | 0.0165 | 3.714 | 0.48 |
0.01 | 5.23 | 1895 | 0.0079 | 4.015 | 0.69 |
0.015 | 5.53 | 2706 | 0.0055 | 5.012 | 0.784 |
0.02 | 5.52 | 2716 | 0.0053 | 5.009 | 0.782 |
0.05 | 5.55 | 2720 | 0.0052 | 5.011 | 0.783 |
0.07 | 4.62 | 1816 | 0.0083 | 4.127 | 0.642 |
0.1 | 4.67 | 1173 | 0.0128 | 4.143 | 0.513 |
Flow rate (mL/minute) | |||||
0.6 | 4.348 | 466 | 0.0322 | 2.11 | 0.92 |
0.7 | 4.291 | 1013 | 0.0148 | 2.64 | 0.84 |
0.8 | 4.342 | 1070 | 0.0141 | 2.69 | 0.75 |
1 | 5.53 | 2706 | 0.0055 | 5.012 | 0.784 |
1.1 | 5.26 | 1982 | 0.0076 | 4.857 | 0.662 |
1.2 | 4.74 | 1486 | 0.0101 | 4.33 | 0.679 |
1.3 | 4.25 | 1057 | 0.0142 | 3.21 | 0.54 |
1.4 | 4.12 | 1013 | 0.0148 | 2.94 | 0.49 |
1.5 | 3.97 | 925 | 0.0162 | 2.76 | 0.33 |