Table 9 Calculated (Q(a,ζ H )/Q(a,ζ D )) and experimental kinetic deuterium isotope effects at 20 C for the hydride transfer catalyzed by Escherichia coli dihydrofolate reductase in various solvents
From: On the curvature in logarithmic plots of rate coefficients for chemical reactions
Solvent | a | KIE a | KIE exp |
|---|---|---|---|
H2O | 1 | 7.15 | 2.70 |
MeOH 17% | 24 | 2.25 | 2.73 |
MeOH 33% | 1 | 7.15 | 2.60 |
MeOH 50% | 1 | 7.15 | 2.70 |
glycerol 17% | 1 | 7.15 | 2.65 |
glycerol 33% | 96 | 1.37 | 2.65 |
glycerol 50% | 169 | 1.23 | 2.66 |
sucrose 17% | 3 | 5.68 | 3.13 |
sucrose 30% | 108 | 1.22 | 3.22 |