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Table 9 Calculated (Q(a,ζ H )/Q(a,ζ D )) and experimental kinetic deuterium isotope effects at 20 C for the hydride transfer catalyzed by Escherichia coli dihydrofolate reductase in various solvents

From: On the curvature in logarithmic plots of rate coefficients for chemical reactions

Solvent a KIE a KIE exp
H2O 1 7.15 2.70
MeOH 17% 24 2.25 2.73
MeOH 33% 1 7.15 2.60
MeOH 50% 1 7.15 2.70
glycerol 17% 1 7.15 2.65
glycerol 33% 96 1.37 2.65
glycerol 50% 169 1.23 2.66
sucrose 17% 3 5.68 3.13
sucrose 30% 108 1.22 3.22
  1. The corresponding TST value is 7.15.
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