Table 2 Summary of purification procedures of recombinant AlEH1.
Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Recovery (%) | Purification (-fold) | |
|---|---|---|---|---|---|
Supernatant | 1,027 | 1,813 | 1.765 | 100 | 1 |
DEAE-Sepharose CL-6B | 55.3 | 1,003 | 18.1 | 55.4 | 10.3 |
Toyopearl HW-55S | 17.3 | 469.5 | 27.1 | 25.9 | 15.4 |
Toyopearl HW-55S | 5.9* | 433.2 | 73.4 | 23.9 | 41.6 |